| Reliability of laboratory markers of HIV-1 infection in Argentinian infants at risk of perinatal infection. | |
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MedLine Citation:
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PMID: 15468443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Early and accurate diagnosis of HIV-1 infection in infants born to HIV-1-seropositive mothers is of great importance. Polymerase chain reaction (PCR), HIV culture, and p24 antigen detection assays were evaluated for their ability to detect the presence of HIV in 195 infants at risk of perinatal infection. Using the Centers for Disease Control and Prevention guidelines for assessing HIV infection status in children younger than 18 months, 70 infants (36%) were diagnosed as HIV-1 infected and 125 (64%) lacked virologic and clinical evidence of infection. PCR and HIV culture were the most sensitive laboratory markers, detecting 100% and 98% of positive samples, respectively, regardless of age at testing. HIV-1 p24 antigen assay was detected in 26 of 38 positive samples but not in negative samples. PCR was performed with three different sets of primers (SK38/SK39-SK19-gag, SK68/SK69-SK70-env, and SK150/SK431-SK102-gag). The sensitivity/specificity of the individual assays were for SK19, 96.1%/94.25%; SK70, 89.6%/100%; and SK102, 100%/100%. A sample was considered HIV-1 positive when two positive PCR results were obtained with two different pairs of primers, and negative if the sample was negative when three sets of primers were used. False-positive results were occasionally obtained with probe SK19 in six seroreverter infants before serologic status was known. This suggested that the infection was caused by nonreplicative strains or were false-positive results probably by nonspecific amplification due to cross-reaction with other microorganisms; contamination was discarded because there was no specific amplification with the other two primers. All the HIV-1-infected infants were correctly identified with PCR; all except one could be identified with coculture and only 68.4% were confirmed with p24 antigen assay. No seroreverter infant was misdiagnosed using the criteria selected. |
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Authors:
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A Mangano; G Pittis; C Galindez; R Bologna; L Sen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: AIDS patient care and STDs Volume: 12 ISSN: 1087-2914 ISO Abbreviation: AIDS Patient Care STDS Publication Date: 1998 Sep |
Date Detail:
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Created Date: 2004-09-17 Completed Date: 2004-10-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9607225 Medline TA: AIDS Patient Care STDS Country: United States |
Other Details:
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Languages: eng Pagination: 691-6 Citation Subset: X |
Affiliation:
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Laboratorio de Biología Celular y Retrovirus, Hospital de Pediatría "Juan P. Garrahan," Buenos Aires, Argentina. labret@anmat.gov.ar |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Argentina
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epidemiology Female HIV Core Protein p24 / analysis HIV Infections / diagnosis*, epidemiology, transmission* HIV-1* / isolation & purification Humans Infant Infant, Newborn Infectious Disease Transmission, Vertical* Male Polymerase Chain Reaction Reproducibility of Results Risk Factors Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/HIV Core Protein p24 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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