Document Detail


Reliability of the hepatocyte primary culture/DNA repair test in testing of coded carcinogens and noncarcinogens.
MedLine Citation:
PMID:  7144803     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hepatocyte primary culture/DNA repair test was evaluated for its reliability using a series of coded samples. Among the 30 chemicals tested, 15 were general reference compounds and 15 were chemicals that had been tested for carcinogenicity in the U.S. National Cancer Institute Bioassay Program. The latter group were from the same lot that had been used for the in vivo testing and had also been tested for mutagenicity in the Ames test. From the group of 15 reference compounds, 5 were positive for DNA repair and all 5 were carcinogens. Of the 10 samples scored as negative, 4 were noncarcinogens and 6 were carcinogens. Among the 6 carcinogens were 3 compounds whose carcinogenicity probably does not involve the production of DNA damage. From the 15 coded chemicals that were tested for carcinogenicity by the NCI in long-term animal studies, 7 were scored as positive. 5 of these were judged carcinogenic in the in vivo bioassays and the other 2, which were also mutagenic in Salmonella, showed some indication of carcinogenicity. Of the 8 compounds that were scored as negative, 5 were noncarcinogenic. Among the 3 carcinogens that were not detected, there was at least one whose carcinogenicity probably does not involve DNA damage. Thus, the results of this study indicate that positive results in the hepatocyte primary culture/DNA repair test are highly specific for carcinogens and that the test is also highly sensitive in the detection of DNA-damaging genotoxic carcinogens.
Authors:
G M Williams; M F Laspia; V C Dunkel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  97     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-01-07     Completed Date:  1983-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  359-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinogens / pharmacology*
Cells, Cultured
DNA Repair / drug effects*
Dose-Response Relationship, Drug
Liver / drug effects*
Male
Rats
Rats, Inbred F344
Research Design
Grant Support
ID/Acronym/Agency:
N0I-CP-55205/CP/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Carcinogens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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