| Reliability of fatty acid composition in human serum phospholipids. | |
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MedLine Citation:
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PMID: 10822282 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: We examined the reliability of the fatty acid composition of serum phospholipids in the New York University Women's Health Study, a prospective study of sex hormones, diet and breast cancer. DESIGN: Non-fasting serum samples collected at three yearly visits, in 46 healthy women, and stored at -80 degrees C for 7-12 y, were included in the study. Serum phospholipid fatty acid composition was measured by capillary gas chromatography. RESULTS: For the 20 individual fatty acids measured, the reliability coefficients were less than 0.50 for four, between 0.50 and 0.70 for nine, and greater than 0.70 for seven. Among the major fatty acids, arachidonic and alpha-linolenic acids had high reliability coefficients (0.71 and 0. 72, respectively), palmitic, oleic, linoleic, eicosapentaenoic and docosahexaenoic acids had intermediate coefficients (0.57, 0.69, 0. 62, 0.64 and 0.66, respectively), whereas stearic acid had the lowest coefficient (0.15). The reliability coefficients for total monounsaturated fats, omega-6 and omega-3 fatty acids were moderately high (0.66, 0.53 and 0.66, respectively), whereas the coefficients for total saturated fats and total polyunsaturated fats were low (0.31 and 0.43, respectively). CONCLUSIONS: These results indicate that the fatty acid composition of serum phospholipids can be a useful tool in epidemiologic studies, although for most fatty acids a single determination is associated with some error in measurement that should be taken into account at the design and analysis stages. Storage for up to 12 y at -80 degrees C preserved polyunsaturated fatty acids from oxidation very well. |
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Authors:
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A Zeleniuch-Jacquotte; V Chajès; A L Van Kappel; E Riboli; P Toniolo |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: European journal of clinical nutrition Volume: 54 ISSN: 0954-3007 ISO Abbreviation: Eur J Clin Nutr Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-06-08 Completed Date: 2000-06-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8804070 Medline TA: Eur J Clin Nutr Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 367-72 Citation Subset: IM |
Affiliation:
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Nelson Institute of Environmental Medicine, New York University Medical Center, New York, NY 10016-3240, USA. jacqua02@popmail.med.nyu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Arachidonic Acid / blood Chromatography, Gas / methods Diet Records Dietary Fats / administration & dosage Fatty Acids / blood* Female Humans Middle Aged Phospholipids / blood* Prospective Studies Quality Control Reproducibility of Results alpha-Linolenic Acid / blood |
| Grant Support | |
ID/Acronym/Agency:
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CA13343/CA/NCI NIH HHS; CA16087/CA/NCI NIH HHS; CA34588/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Fatty Acids; 0/Phospholipids; 463-40-1/alpha-Linolenic Acid; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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