| Reliability of Nucleus-To-Cell and Nucleus-To-Cytoplasm Calculations for Conjunctival Impression Cytology Specimens. | |
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MedLine Citation:
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PMID: 22574637 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose: To assess the outcome of two different nucleo-cytoplasmic ratio calculations in relation to the number of cells measured from conjunctival impression cytology (CIC) images. Methods: CIC from the exposed nasal bulbar conjunctiva surface was undertaken on 40 young adults using a Millcell(®)-CM filter; the filters were stained with Giemsa, colour images taken of the cells in a monolayer at 200 X magnification, graded by the Nelson scale and then a projection overlay method used to outline the cell and nucleus borders. Nucleo-cytoplasmic ratios were calculated based on literal interpretation of the term (i.e. N/CYT = nucleus area/[cell area - nucleus area]) as well as based on cell area (i.e. N/CELL = nucleus area/cell area). Results: A range of N/CYT or N/CELL values are expected for all cells, regardless of squamous metaplasia grade, with slightly greater heterogeneity expected from N/CYT calculations. The difference between the two calculations gets progressively less as the squamous metaplasia grade increases. For all grades of cells, these ratios could be determined to within approximately ±1% if adequate numbers of cells were measured (i.e. 75, 65, 50 and 35 for grade 0 to grade 3 cells respectively). If fewer cells are measured, then the variability in N/CELL or N/CYT calculations can be expected to increase. Conclusions: In assessments of nucleo-cytoplasmic ratios, it is important that the calculation used be specified and that the numbers of cells measured be provided. |
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Authors:
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Michael J Doughty |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-10 |
Journal Detail:
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Title: Current eye research Volume: - ISSN: 1460-2202 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Glasgow-Caledonian University, Department of Vision Sciences , Glasgow , Scotland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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