Document Detail

Relevance of serology for Mycoplasma pneumoniae diagnosis compared with PCR and culture in acute exacerbation of bronchial asthma.
MedLine Citation:
PMID:  19095568     Owner:  NLM     Status:  MEDLINE    
We studied Mycoplasma pneumoniae as the etiologic pathogen in acute exacerbations of asthma and the value of polymerase chain reaction (PCR), culture, and serologic tests for its accurate diagnosis. For the study, 59 nonsmoking patients with asthma (37 females, 22 males; age, 15-50 years) underwent clinical, radiologic, and laboratory examinations. Bacteria isolated from sputum were Streptococcus pneumoniae (32 [54%]), Staphylococcus aureus (23 [39%]), and M pneumoniae (5 [15%]). All M pneumoniae were associated with S pneumoniae (8/32 [25%]) and S aureus (1/23 [4%]). No M pneumoniae were isolated as single pathogens. Serologic testing for M pneumoniae revealed that all samples were positive for specific IgG; 40 (68%) had a high titer, and 19 (32%) had a moderate titer. Of 59 samples, 29 (49%) were positive by Serodia Myco II gelatin particle testing (Fujirebio, Tokyo, Japan). PCR was positive in 25 samples, all of which had a high IgG titer; all culture-positive cases were PCR+. M pneumoniae is a common bacterial pathogen associated with acute exacerbations of asthma in people 15 years or older. Prompt laboratory diagnosis of M pneumoniae requires direct detection by PCR and culture. A high serologic titer can be a clue for the presence of M pneumoniae.
Maysaa El Sayed Zaki; Doaa Raafat; Amal Abd El Metaal
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  131     ISSN:  1943-7722     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-19     Completed Date:  2009-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  74-80     Citation Subset:  AIM; IM    
Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
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MeSH Terms
Asthma / diagnosis,  microbiology*
Middle Aged
Mycoplasma pneumoniae / immunology*
Pneumonia, Mycoplasma / diagnosis*
Polymerase Chain Reaction
Serologic Tests
Sputum / microbiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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