| Release of ouabain-like compound (OLC) from the intact perfused rat adrenal gland. | |
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MedLine Citation:
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PMID: 9888565 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study was designed to investigate the effect of ACTH on release of OLC from the intact isolated rat adrenal, perfused in situ. OLC was measured by EIA. The limit of detection of the assay was 23 pmol/10 minutes. Basal levels of OLC varied from 240 to <23 pmol/10 min. Basal corticosterone levels were generally higher than OLC while aldosterone were generally lower. In 3 of the 5 perfusions the addition of ACTH was followed by rapid increases in both OLC and corticosterone secretion rates within 10 minutes of stimulation. Stimulated levels of OLC were 2 to 4-fold and of corticosterone were 3 to 7-fold those found in basal samples. OLC was found to co-elute with authentic ouabain under isocratic HPLC analysis whilst perfusion medium itself contained no detectable OLC immunoreactivity. These preliminary data suggest that the intact perfused rat adrenal preparation is a useful model for investigating the acute regulation of OLC secretion. |
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Authors:
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J P Hinson; S Harwood; A B Dawnay |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Endocrine research Volume: 24 ISSN: 0743-5800 ISO Abbreviation: Endocr. Res. Publication Date: 1998 Aug-Nov |
Date Detail:
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Created Date: 1999-04-13 Completed Date: 1999-04-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8408548 Medline TA: Endocr Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 721-4 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, St Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Glands
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drug effects,
metabolism* Adrenocorticotropic Hormone / pharmacology* Aldosterone / secretion Animals Chromatography, High Pressure Liquid Corticosterone / secretion Culture Techniques Male Ouabain / metabolism* Perfusion Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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50-22-6/Corticosterone; 52-39-1/Aldosterone; 630-60-4/Ouabain; 9002-60-2/Adrenocorticotropic Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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