| Release of inflammatory mediators from eosinophils following a hyperosmolar stimulus. | |
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MedLine Citation:
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PMID: 12924520 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction (EIB). It has been shown that an in vitro hyperosmolar stimulation of basophils and mast cells with mannitol can induce the release of histamine and leukotrienes. The aim of this study was to establish if a hyperosmolar challenge could trigger activation of eosinophils to release chemokines and lipid mediators. Peripheral blood eosinophils were isolated from seven asthmatic and six non-asthmatic subjects. Hyperosmolar stimulation of eosinophils with mannitol (0.7 M), resulted in a significant increase in LTC4 levels compared to baseline in both asthmatic (15.2+/-4.6 vs. 70.1+/-9.5; P = 0.0002) and control subjects (14.3+/-4.0 vs. 55.6+/-5.6; P = 0.0001). ECP levels did not increase significantly above baseline following mannitol stimulation in either group. This study shows that eosinophils can be activated by a hyperosmolar stimulus. Therefore it seems reasonable to suggest that eosinophils could contribute to EIB. |
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Authors:
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E D Moloney; S Griffin; C M Burke; L W Poulter; S O'Sullivan |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Respiratory medicine Volume: 97 ISSN: 0954-6111 ISO Abbreviation: Respir Med Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-08-19 Completed Date: 2003-09-26 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8908438 Medline TA: Respir Med Country: England |
Other Details:
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Languages: eng Pagination: 928-32 Citation Subset: IM |
Affiliation:
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Department of Respiratory Medicine, James Connolly Memorial Hospital, Dublin, Ireland. e.moloney@ic.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Asthma / metabolism*, pathology Blood Proteins / metabolism Bronchoconstriction / physiology Diuretics, Osmotic / pharmacology Enzyme-Linked Immunosorbent Assay Eosinophil Granule Proteins Eosinophils / metabolism* Exercise Female Humans Inflammation Mediators / metabolism* Leukotriene C4 / metabolism Male Mannitol / pharmacology Middle Aged Osmolar Concentration Ribonucleases* |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Diuretics, Osmotic; 0/Eosinophil Granule Proteins; 0/Inflammation Mediators; 69-65-8/Mannitol; 72025-60-6/Leukotriene C4; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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