Document Detail

Release of inflammatory mediators from eosinophils following a hyperosmolar stimulus.
MedLine Citation:
PMID:  12924520     Owner:  NLM     Status:  MEDLINE    
Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction (EIB). It has been shown that an in vitro hyperosmolar stimulation of basophils and mast cells with mannitol can induce the release of histamine and leukotrienes. The aim of this study was to establish if a hyperosmolar challenge could trigger activation of eosinophils to release chemokines and lipid mediators. Peripheral blood eosinophils were isolated from seven asthmatic and six non-asthmatic subjects. Hyperosmolar stimulation of eosinophils with mannitol (0.7 M), resulted in a significant increase in LTC4 levels compared to baseline in both asthmatic (15.2+/-4.6 vs. 70.1+/-9.5; P = 0.0002) and control subjects (14.3+/-4.0 vs. 55.6+/-5.6; P = 0.0001). ECP levels did not increase significantly above baseline following mannitol stimulation in either group. This study shows that eosinophils can be activated by a hyperosmolar stimulus. Therefore it seems reasonable to suggest that eosinophils could contribute to EIB.
E D Moloney; S Griffin; C M Burke; L W Poulter; S O'Sullivan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Respiratory medicine     Volume:  97     ISSN:  0954-6111     ISO Abbreviation:  Respir Med     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-19     Completed Date:  2003-09-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8908438     Medline TA:  Respir Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  928-32     Citation Subset:  IM    
Department of Respiratory Medicine, James Connolly Memorial Hospital, Dublin, Ireland.
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MeSH Terms
Asthma / metabolism*,  pathology
Blood Proteins / metabolism
Bronchoconstriction / physiology
Diuretics, Osmotic / pharmacology
Enzyme-Linked Immunosorbent Assay
Eosinophil Granule Proteins
Eosinophils / metabolism*
Inflammation Mediators / metabolism*
Leukotriene C4 / metabolism
Mannitol / pharmacology
Middle Aged
Osmolar Concentration
Reg. No./Substance:
0/Blood Proteins; 0/Diuretics, Osmotic; 0/Eosinophil Granule Proteins; 0/Inflammation Mediators; 69-65-8/Mannitol; 72025-60-6/Leukotriene C4; EC 3.1.-/Ribonucleases

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