| Release of glucagon-like peptide 1 immunoreactivity from the perfused rat pancreas. | |
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MedLine Citation:
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PMID: 3577586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A reliable radioimmunoassay for glucagon-like peptide 1 (GLP-1) was developed with a detection limit of 12 pmol/l, which enabled us to detect the subtle change in concentrations of this peptide in the perfusate from the perfused rat pancreas. With this RIA, GLP-1 immunoreactivity (GLP-1 IR) was found to be secreted synchronously with glucagon immunoreactivity upon arginine stimulation from the perfused rat pancreas. Gel chromatographic analysis showed the presence of GLP-1 IR in the pancreatic extract which is eluted at the same position as synthetic GLP-1 (1-37). Moreover HPLC analysis confirmed the presence of GLP-1 IR, which eluted at exactly the same position as synthetic GLP-1, in the perfusate from pancreas perfusion upon stimulation of arginine. These results suggest that the pancreas stores and secrets GLP-1 IR concomitantly with glucagon as one of the cleavage products of their common precursor. |
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Authors:
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K Shima; M Hirota; C Ohboshi; M Sato; T Nishino |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta endocrinologica Volume: 114 ISSN: 0001-5598 ISO Abbreviation: Acta Endocrinol. Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-06-10 Completed Date: 1987-06-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370312 Medline TA: Acta Endocrinol (Copenh) Country: DENMARK |
Other Details:
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Languages: eng Pagination: 531-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / pharmacology* Chromatography, Gel Chromatography, High Pressure Liquid Glucagon / analysis, secretion* Glucagon-Like Peptide 1 Male Pancreas / drug effects* Peptides / analysis, secretion* Perfusion Radioimmunoassay Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 74-79-3/Arginine; 89750-14-1/Glucagon-Like Peptide 1; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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