| Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis. | |
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MedLine Citation:
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PMID: 20880516 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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OBJECTIVE: The emergence of periodontal medicine increased interest in defining the behaviour of peripheral blood cells in periodontitis subjects in comparison with healthy group. The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factor-α (TNF-α), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic periodontitis subjects. DESIGN: PBMC samples were isolated from 19 systemically healthy donors, divided into generalized chronic periodontitis (n=10) and healthy (n=9) subjects. Cells were incubated for 24-48 h in 500 μL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of IL-8, TNF-α, IL-6 and IL-10 released using enzyme-linked immunosorbent assay (ELISA). RESULTS: PBMC cells from periodontitis subjects released higher levels of TNF-α and IL-6 than those from healthy subjects (P<0.05). Conversely, the supernatants of the stimulated PBMC cells obtained from healthy subjects presented higher amounts of IL-8 than those from periodontitis (P<0.05). No differences were observed in the levels of IL-10 (P>0.05) between groups. CONCLUSION: In conclusion, the results of the present study showed that E. coli LPS-stimulated PBMC from subjects with periodontitis present a different pattern of cytokine release when compared to PBMC from healthy subjects. This phenomenon could have implications locally, in periodontitis, as well as in systemic diseases. |
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Authors:
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Thais Oliveira Gonçalves; Dirceu Costa; Claudia Ida Brodskyn; Poliana Mendes Duarte; João Batista César Neto; Getulio Nogueira-Filho |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-28 |
Journal Detail:
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Title: Archives of oral biology Volume: 55 ISSN: 1879-1506 ISO Abbreviation: Arch. Oral Biol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: England |
Other Details:
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Languages: eng Pagination: 975-80 Citation Subset: D; IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Prosthodontics and Periodontics, Faculty of Dentistry of Piracicaba, UNICAMP, Piracicaba, São Paulo, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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