| Release of a chimeric protein into the medium from Escherichia coli using the C-terminal secretion signal of haemolysin. | |
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MedLine Citation:
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PMID: 3119330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, we have identified a novel topogenic sequence at the C terminus of Escherichia coli haemolysin (HlyA) which is essential for its efficient secretion into the medium. This discovery has introduced the possibility of using this secretion system for the release of chimeric proteins from E. coli directly into the medium. We have now successfully fused this C-terminal signal to a hybrid protein containing a few residues of beta-galactosidase and the majority of the E. coli outer membrane porin OmpF lacking its own N-terminal signal sequence. We find that this chimeric protein is specifically translocated across the inner and outer membranes and is released into the medium. In addition, we have further localized the HlyA secretion signal to the final 113 amino acids of the C terminus. In fact, a specific secretion signal appears to reside at least in part within the last 27 amino acids of HlyA. |
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Authors:
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N Mackman; K Baker; L Gray; R Haigh; J M Nicaud; I B Holland |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The EMBO journal Volume: 6 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1987 Sep |
Date Detail:
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Created Date: 1988-01-11 Completed Date: 1988-01-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2835-41 Citation Subset: IM |
Affiliation:
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Research Institute of Scripps Clinic, Department of Immunology, La Jolla, CA 92037. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacterial Outer Membrane Proteins / genetics Chimera* Escherichia coli / genetics*, metabolism Genes Genes, Bacterial Hemolysin Proteins / genetics*, metabolism Molecular Sequence Data Plasmids beta-Galactosidase / genetics |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Hemolysin Proteins; EC 3.2.1.23/beta-Galactosidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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