Document Detail

Release of alpha-subunit of glycoprotein hormones from the bullfrog pituitary: possible effect of alpha-subunit on prolactin cell function.
MedLine Citation:
PMID:  8860318     Owner:  NLM     Status:  MEDLINE    
On the basis of our previous finding that prolactin (PRL) cells of the bullfrog (Rana catesbeiana) contain immunoreactive alpha-subunit of pituitary glycoprotein hormones, experiments were conducted to see whether the immunoreactive alpha-subunit is released from PRL cells. Cell immunoblot analysis revealed that approximately 10% of cells that released immunoreactive PRL also released immunoreactive alpha-subunit. Western blot analysis of the culture media revealed that a considerable amount of the free form of alpha-subunit was released from the dispersed anterior pituitary cells. Moreover, it was found that addition of alpha-subunit to the culture medium enhanced PRL release. The results raise the possibility that alpha-subunit secreted by PRL cells acts as an autocrine and/or paracrine factor.
A Oguchi; S Tanaka; K Yamamoto; S Kikuyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  102     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-03-11     Completed Date:  1997-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  141-6     Citation Subset:  IM    
Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjuku-ku, Tokyo, Japan.
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MeSH Terms
Blotting, Western
Glycoproteins / chemistry*
Peptide Fragments / secretion*
Pituitary Gland, Anterior / secretion*
Pituitary Hormones / chemistry*
Rana catesbeiana / physiology*
Reg. No./Substance:
0/Glycoproteins; 0/Peptide Fragments; 0/Pituitary Hormones

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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