| Release of alpha-subunit of glycoprotein hormones from the bullfrog pituitary: possible effect of alpha-subunit on prolactin cell function. | |
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MedLine Citation:
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PMID: 8860318 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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On the basis of our previous finding that prolactin (PRL) cells of the bullfrog (Rana catesbeiana) contain immunoreactive alpha-subunit of pituitary glycoprotein hormones, experiments were conducted to see whether the immunoreactive alpha-subunit is released from PRL cells. Cell immunoblot analysis revealed that approximately 10% of cells that released immunoreactive PRL also released immunoreactive alpha-subunit. Western blot analysis of the culture media revealed that a considerable amount of the free form of alpha-subunit was released from the dispersed anterior pituitary cells. Moreover, it was found that addition of alpha-subunit to the culture medium enhanced PRL release. The results raise the possibility that alpha-subunit secreted by PRL cells acts as an autocrine and/or paracrine factor. |
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Authors:
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A Oguchi; S Tanaka; K Yamamoto; S Kikuyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: General and comparative endocrinology Volume: 102 ISSN: 0016-6480 ISO Abbreviation: Gen. Comp. Endocrinol. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1997-03-11 Completed Date: 1997-03-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 141-6 Citation Subset: IM |
Affiliation:
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Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjuku-ku, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Female Glycoproteins / chemistry* Immunohistochemistry Peptide Fragments / secretion* Pituitary Gland, Anterior / secretion* Pituitary Hormones / chemistry* Rana catesbeiana / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Peptide Fragments; 0/Pituitary Hormones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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