| Release kinetics of polymer-bound bone morphogenetic protein-2 and its effects on the osteogenic expression of MC3T3-E1 osteoprecursor cells. | |
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MedLine Citation:
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PMID: 19337085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In an effort to augment scaffold performance, additives such as growth factors are under investigation for their ability to optimize the "osteopotential" of synthetic polymer scaffolds. In parallel research, bone morphogenetic protein-2 (BMP-2), a growth factor that initiates bone formation, has been locally delivered to augment fracture healing and spinal fusion. The authors hypothesize that BMP-2 can be covalently bound to a polymer substrate, increasing its concentration and bioavailability over longer periods, thus improving the efficacy of the growth factor and subsequently the bony matrix production. It would remain bound longer when compared with published controls. This prolonged binding would then increase the bioavailability of the growth factor and thus increase bony matrix production over a longer interval. METHODS: Mouse preosteoblast MC3T3-E1 cells were cultured on poly(lactic-co-glycolic acid) and polycaprolactone polymer disks covalently bound with BMP-2 to assess the progression and quality of osteogenesis. Covalent binding of BMP-2 to each polymer was visualized by immunohistochemical analysis of polymer-coated microscope slides. The quantity of covalently bound BMP-2 was determined using enzyme-linked immunosorbent assay. RESULTS: Polymerase chain reaction results showed elevated expression levels for alkaline phosphatase and osteocalcin genes. BMP-2 was released from polycaprolactone over 2 weeks, with 86 percent remaining covalently bound, in contrast to 93 percent retained by poly(lactic-co-glycolic acid). CONCLUSIONS: BMP-2, proven to alter polymer osteogenicity, remained bound to poly(lactic-co-glycolic acid), which may render poly(lactic-co-glycolic acid) an ideal choice as a polymer for scaffold-based bone tissue engineering using growth factor delivery. |
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Authors:
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Nareg A Gharibjanian; Walter C Chua; Sanjay Dhar; Thomas Scholz; Terry Y Shibuya; Gregory R D Evans; Jay W Calvert |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Plastic and reconstructive surgery Volume: 123 ISSN: 1529-4242 ISO Abbreviation: Plast. Reconstr. Surg. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-01 Completed Date: 2009-05-05 Revised Date: 2011-02-16 |
Medline Journal Info:
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Nlm Unique ID: 1306050 Medline TA: Plast Reconstr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1169-77 Citation Subset: AIM; IM |
Affiliation:
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Aesthetic and Plastic Surgery Institute, Orange, Calif., USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Bone Morphogenetic Protein 2 / analysis, pharmacokinetics* Cells, Cultured Glycolates* Mice Osteogenesis* Polyesters* |
| Chemical | |
Reg. No./Substance:
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0/Bone Morphogenetic Protein 2; 0/Glycolates; 0/Polyesters; 0/polylactic acid-polyglycolic acid copolymer; 24980-41-4/polycaprolactone |
| Comments/Corrections | |
Comment In:
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Plast Reconstr Surg. 2009 Dec;124(6):2199-200; author reply 2200
[PMID:
19952695
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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