Document Detail


Relaxin stimulates cAMP production in MCF-7 cells upon overexpression of type V adenylyl cyclase.
MedLine Citation:
PMID:  15956721     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Relaxin stimulates cAMP production and activation of ERK and PI3K in THP-1 cells. Relaxin also stimulates protein kinase C zeta (PKCzeta) translocation to the plasma membrane in a PI3K-dependent manner in THP-1 and MCF-7 cells. However, relaxin did not increase cAMP production in MCF-7 cells. We overexpressed different adenylyl cyclase (AC) isoforms in MCF-7 cells to examine coupling of endogenous relaxin receptors to cAMP production. Overexpression of types II and IV AC had no effect on cAMP production by relaxin. However, overexpression of type V AC, which is activated by PKCzeta, showed synergistic stimulation of cAMP by relaxin and forskolin.
Authors:
Bao T Nguyen; Carmen W Dessauer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1041     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-06-15     Completed Date:  2005-10-26     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  296-9     Citation Subset:  IM    
Affiliation:
Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, TX 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / classification*,  genetics,  metabolism*
Cell Line, Tumor
Cyclic AMP / biosynthesis*
Gene Expression*
Humans
Relaxin / pharmacology*
Grant Support
ID/Acronym/Agency:
GM60419/GM/NIGMS NIH HHS; R01 GM060419-05/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
60-92-4/Cyclic AMP; 9002-69-1/Relaxin; EC 4.6.1.1/Adenylate Cyclase
Comments/Corrections

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