| Relative retention order of all isomers of cis/trans conjugated linoleic acid FAME from the 6,8- to 13,15-positions using silver ion HPLC with two elution systems. | |
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MedLine Citation:
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PMID: 16094861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CLA, defined as one or more octadecadienoic acids (18:2) with conjugated double bonds, has been reported to be active in a number of biological systems. GC and silver ion HPLC (Ag(+)-HPLC) have been the primary techniques for identifying specific CLA isomers in both foods and biological extracts. Recently, GC relative retention times were reported for all c,c, c/t (c,t and tc), and t,t CLA FAME from the 6,8- to the 13,15-positions in octadecadienoic acid (18:2). Presented here is the relative retention order of the same CLA FAME using Ag(+)-HPLC with two different elution systems. The first elution system, consisting of 0.1% acetonitrile/0.5% diethyl ether (DE)/hexane, has been used previously to monitor CLA composition in foods. Also presented here is the retention order of CLA FAME using 2% acetic acid/hexane elution solvent, which has advantages of more stable retention volumes and a complementary elution order of CLA FAME isomers. The data are reported using retention volumes (RV) adjusted for toluene, an estimator for dead volume, and relative to c9,t11-18:2. Measurement of relative RV in the analysis of 88 samples of cow plasma, milk, and rumen fluids using Ag(+)-HPLC is also presented here. The % CV ranged from 1.04 to 1.62 for t,t isomers and from 0 to 0.48 for c/t isomers. |
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Authors:
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Pierluigi Delmonte; Ai Kataok; B A Corl; D E Bauman; Martin P Yurawecz |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Lipids Volume: 40 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-08-12 Completed Date: 2005-09-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 509-14 Citation Subset: IM |
Affiliation:
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US Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, Maryland 20740, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetonitriles Animals Body Fluids / chemistry Cattle Chromatography, High Pressure Liquid / methods* Ether, Ethyl Isomerism Linoleic Acids, Conjugated / blood, isolation & purification* Milk / chemistry* Reproducibility of Results Rumen / chemistry Silver |
| Chemical | |
Reg. No./Substance:
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0/Acetonitriles; 0/Linoleic Acids, Conjugated; 60-29-7/Ether, Ethyl; 7440-22-4/Silver; 75-05-8/acetonitrile |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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