Document Detail


Relative retention order of all isomers of cis/trans conjugated linoleic acid FAME from the 6,8- to 13,15-positions using silver ion HPLC with two elution systems.
MedLine Citation:
PMID:  16094861     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CLA, defined as one or more octadecadienoic acids (18:2) with conjugated double bonds, has been reported to be active in a number of biological systems. GC and silver ion HPLC (Ag(+)-HPLC) have been the primary techniques for identifying specific CLA isomers in both foods and biological extracts. Recently, GC relative retention times were reported for all c,c, c/t (c,t and tc), and t,t CLA FAME from the 6,8- to the 13,15-positions in octadecadienoic acid (18:2). Presented here is the relative retention order of the same CLA FAME using Ag(+)-HPLC with two different elution systems. The first elution system, consisting of 0.1% acetonitrile/0.5% diethyl ether (DE)/hexane, has been used previously to monitor CLA composition in foods. Also presented here is the retention order of CLA FAME using 2% acetic acid/hexane elution solvent, which has advantages of more stable retention volumes and a complementary elution order of CLA FAME isomers. The data are reported using retention volumes (RV) adjusted for toluene, an estimator for dead volume, and relative to c9,t11-18:2. Measurement of relative RV in the analysis of 88 samples of cow plasma, milk, and rumen fluids using Ag(+)-HPLC is also presented here. The % CV ranged from 1.04 to 1.62 for t,t isomers and from 0 to 0.48 for c/t isomers.
Authors:
Pierluigi Delmonte; Ai Kataok; B A Corl; D E Bauman; Martin P Yurawecz
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  40     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-08-12     Completed Date:  2005-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  509-14     Citation Subset:  IM    
Affiliation:
US Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, Maryland 20740, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetonitriles
Animals
Body Fluids / chemistry
Cattle
Chromatography, High Pressure Liquid / methods*
Ether, Ethyl
Isomerism
Linoleic Acids, Conjugated / blood,  isolation & purification*
Milk / chemistry*
Reproducibility of Results
Rumen / chemistry
Silver
Chemical
Reg. No./Substance:
0/Acetonitriles; 0/Linoleic Acids, Conjugated; 60-29-7/Ether, Ethyl; 7440-22-4/Silver; 75-05-8/acetonitrile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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