Document Detail


Relative resistance of the F-42-stabilized classical pathway C3 convertase to inactivation by C4-binding protein.
MedLine Citation:
PMID:  6903579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sera of some patients with SLE contain an IgG antibody (F-42) directed against the classical pathway C3 convertase (C-42), which is capable of stabilizing C42 in a dose-dependent manner. The half-life (T 1/2) of C42 is prolonged by F-42. In order to determine whether C4-binding protein was capable of reversing stabilization of C42, stabilized and unstabilized cell-bound C42 were exposed to purified C4-bp and the convertase activity was assessed. C4-bp was capable of accelerating the decay of C42 in a dose-dependent manner; 2 microgram/ml C4-bp reduced the T 1/2 of C42 from 5 to 2.5 min at 30 degrees C. On the other hand, 16 microgram C4-bp could reverse stabilization of C42 by F-42 from T 1/2 = 78 min to a T 1/2 - 40 min; 128 microgram C4-bp reduced the T 1/2 of stabilized C42 to 4 min. Functional inactivation of C42 occurs via enhanced decay-dissociation of C2 from the convertase by C4-bp, as shown by the release of 125I-C2i from the cell-bound convertase. Stabilization of C42 by F-42 is caused by prevention of decay-dissociation of 125I-C2. F-42 was also capable of stabilizing C4oxy2 even further, as shown by prolongation of the T 1/2 of cell-bound C4 oxy2 to a T 1/2 of at least 300 min at 30 degrees C.
Authors:
M R Daha; L A van Es
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  125     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1980 Nov 
Date Detail:
Created Date:  1981-01-29     Completed Date:  1981-01-29     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2051-4     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies
Carrier Proteins*
Complement Activating Enzymes*
Complement Activation*
Complement C2
Complement C3-C5 Convertases*
Complement C4*
Complement Pathway, Classical*
Dose-Response Relationship, Immunologic
Humans
Chemical
Reg. No./Substance:
0/Antibodies; 0/Carrier Proteins; 0/Complement C2; 0/Complement C4; EC 3.-/Complement Activating Enzymes; EC 3.4.21.-/Complement C3-C5 Convertases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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