Document Detail


Relative influence of unfrozen fraction and salt concentration on the survival of slowly frozen eight-cell mouse embryos.
MedLine Citation:
PMID:  3816286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This laboratory has previously reported that the survival of frozen-thawed human erythrocytes is determined more by the fraction of the extracellular solution that remains unfrozen than by the salt concentration in that fraction, especially when the cells are frozen at low hematocrit. To determine the extent to which these findings are applicable to nucleated mammalian cells, we have studied the survival of some 3300 mouse embryos as a function of the unfrozen fraction and the concentration of salt in that unfrozen fraction. Also varied in the study was the weight percentage ratio of glycerol to salt. The concentration of embryos in these experiments (i.e., the cytocrit) was so low that embryo-embryo contacts should have been rare during the freezing. As in the case of the red cells at low hematocrit, we find that the survival of slowly frozen eight-cell embryos is not affected by the high concentrations of salt produced by freezing, at least up to 3.3 molal NaCl, and therefore is not affected by the extent to which the cells shrink below their isotonic volume, nor in general is survival influenced by the temperature at which given salt concentrations and unfrozen fractions are attained or by the glycerol concentration at those temperatures. On the other hand, the attainment of low values of the unfrozen fraction (U) is damaging, but the damage appears in part to be due to the fact that low values of U had to be achieved by placing embryos in solutions hypotonic with respect to NaCl, which caused their volume to be greater than isotonic prior to freezing.
Authors:
U Schneider; P Mazur
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cryobiology     Volume:  24     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-04-14     Completed Date:  1987-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology*
Cell Survival
Freezing
Glycerol
Mice
Osmolar Concentration
Sodium Chloride
Temperature
Chemical
Reg. No./Substance:
56-81-5/Glycerol; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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