| Relative importance of newly synthesized and stored hormone to basal secretion by growth hormone and prolactin cells. | |
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MedLine Citation:
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PMID: 2507285 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is generally accepted that under basal conditions there is preferential release of newly synthesized hormone by a number of endocrine cell type, including those that secrete GH or PRL. However, the cellular basis for this phenomenon along with the relative contribution of stored hormone to basal secretion has yet to be clearly established. In the present study, we employed reverse hemolytic plaque assays to monitor basal and stimulated release of GH and PRL from individual cells in which de novo protein synthesis had been blocked. Monodispersed pituitaries from adult male rats were cultured for 21 h in the absence or presence of maximally effective doses of puromycin (100 microM) or cycloheximide (36 microM) and were then subjected to separate plaque assays for GH or PRL. Treatment with puromycin reduced the percentage of GH or PRL secretors (plaque formers) by about half. Coincubation with stimulatory secretagogues did not increase the percentages of GH or PRL secretors in control cultures, but returned the proportion in puromycin-treated cells to normal, demonstrating that cells which failed to secrete basally could still release hormone from their stored pools when stimulated. Very similar results were obtained when these experiments were repeated with cycloheximide. Taken together, these results demonstrate that only a fraction of the cells that release GH or PRL are dependent upon newly synthesized hormone for basal secretion; the remainder appear capable of mobilizing stored hormone for this purpose even in the absence of stimulation. |
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Authors:
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T T Chen; R D Kineman; J G Betts; J B Hill; L S Frawley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 125 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1989-11-07 Completed Date: 1989-11-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1904-9 Citation Subset: AIM; IM |
Affiliation:
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Department of Anatomy and Cell Biology, Medical University of South Carolina, Charleston 29425. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Cycloheximide / pharmacology Growth Hormone / metabolism*, secretion Growth Hormone-Releasing Hormone / pharmacology Male Pituitary Gland / cytology, metabolism*, secretion Prolactin / biosynthesis, metabolism*, secretion Protein Biosynthesis Proteins / antagonists & inhibitors, secretion Puromycin / pharmacology Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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DK-38215/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 53-79-2/Puromycin; 66-81-9/Cycloheximide; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone; 9034-39-3/Growth Hormone-Releasing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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