Document Detail


Relative diversity and community structure analysis of rumen protozoa according to T-RFLP and microscopic methods.
MedLine Citation:
PMID:  22033497     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Protozoa are common inhabitants of the rumen where they play roles in host nutrition and methanogenesis. Knowledge of how changes in the composition of protozoa communities affect these processes is limited in part due to a lack of efficient methods for protozoa community analysis. In this study, a terminal-restriction fragment length polymorphism (T-RFLP) assay targeting the 18S rRNA gene was developed for comparative analysis of rumen protozoa communities. Comparison of diversity and structure of protozoa communities from hay-fed versus silage/grain-fed cattle via T-RFLP analysis yielded similar overall results to microscopy analysis. According to both methods, Entodinium spp. were more abundant in the silage/grain-fed cattle and protozoa diversity (as calculated using the Shannon index) was higher for the hay-fed cattle due to greater species evenness. Type B protozoa were more prevalent in the hay-fed cattle, whereas Type A protozoa were more prevalent in the silage/grain-fed cattle. Analysis of similarity (ANOSIM) indicated that the protozoa communities from hay-fed and silage/grain-fed cattle were different, and multivariate analysis indicated that pen mates (i.e., cattle fed the same diet and housed together) tended to have similar protozoa communities types. In summary, we present a T-RFLP method for analyzing rumen protozoa communities which complements traditional microscopy approaches but has the advantage of being amenable to high-throughput.
Authors:
Lisa Tymensen; Cindy Barkley; Tim A McAllister
Related Documents :
19779207 - Modified atkins diet for the treatment of nonconvulsive status epilepticus in children.
16009297 - An educational model for improving diet counselling in primary care a case study of the...
18358107 - Multiple symmetric lipomatosis type 2 in females--report of two cases.
20833077 - Anti-inflammatory properties of high-density lipoprotein cholesterol in chronic hemodia...
15258377 - Effect of a hypocaloric diet on lipids and rheological profile in subjects with severe ...
11510487 - Effect of dexamethasone palmitate-low density lipoprotein complex on cholesterol ester ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-18
Journal Detail:
Title:  Journal of microbiological methods     Volume:  -     ISSN:  1872-8359     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Objective markers of drug effects on brain function from recordings of scalp potential in healthy vo...
Next Document:  High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type...