Document Detail

Relative differences in aryl hydrocarbon receptor-mediated response for 18 polybrominated and mixed halogenated dibenzo-p-dioxins and -furans in cell lines from four different species.
MedLine Citation:
PMID:  17941736     Owner:  NLM     Status:  MEDLINE    
As a consequence of ubiquitous use of brominated organic chemicals, there is a concern for persistent or increasing environmental levels of polybrominated dibenzo-p-dioxins/furans (PBDD/Fs) and mixed polychlorinated and polybrominated dibenzo-p-dioxins/furans (PXDD/Fs). Hence, there is a need to broaden the toxicological and environmental knowledge about these compounds, as a basis for risk assessment. In the study presented here, the relative potencies (REPs) for 18 PBDD/F and PXDD/ F congeners were determined in four dioxin-specific bioassays from different species: dioxin receptor chemically activated luciferase expression assay (DR-CALUX, rat hepatoma cells), TV101L (human hepatoma cells), and GPC.2D (guinea pig adenoma cells), as well as ethoxyresorufin-O-deethylase induction in the fish cell line RTL-W1 (rainbow trout liver cells). The bioassay specific REP factors presented here enable the assessment of the contribution from PBDD/Fs and PXDD/Fs to total 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TEQs: toxic equivalents), using bioassay analysis. The PBDD/Fs were found to be equally potent as their chlorinated analogues in the three mammalian assays, whereas the PXDD/Fs showed relatively higher potencies. Of special concern were the 2,3,7,8-substituted penta- and tetrahalogenated congeners, for which mean REPs were > or =1. The 2-B-1,3,7,8-CDD (2-bromo-1,3,7,8-tetrachlorodibenzo-p-dioxin) was up to three times more potent than TCDD in individual experiments (on weight basis). The RTL-W1 was less sensitive to the tested compounds with overall 10-fold lower REPs than the mammalian cell lines. Although the REP factors exhibited species-specific differences, overall resembling rank orders of dioxin-like potency were obtained.
Helena Olsman; Magnus Engwall; Ulrike Kammann; Martin Klempt; Jens Otte; Bert van Bavel; Henner Hollert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental toxicology and chemistry / SETAC     Volume:  26     ISSN:  0730-7268     ISO Abbreviation:  Environ. Toxicol. Chem.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-18     Completed Date:  2008-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8308958     Medline TA:  Environ Toxicol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2448-54     Citation Subset:  IM    
Man-Technology-Environment Research Centre (MTM), Department of Natural Science, University of Orebro, Orebro, Sweden.
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MeSH Terms
Adenoma / pathology
Biological Assay
Cell Line
Dioxins / toxicity*
Environmental Pollutants / toxicity*
Furans / toxicity*
Guinea Pigs
Hydrocarbons, Brominated / toxicity
Hydrocarbons, Halogenated / toxicity*
Liver / drug effects*,  pathology
Oncorhynchus mykiss
Receptors, Aryl Hydrocarbon / metabolism*
Risk Assessment
Tetrachlorodibenzodioxin / toxicity
Reg. No./Substance:
0/Dioxins; 0/Environmental Pollutants; 0/Furans; 0/Hydrocarbons, Brominated; 0/Hydrocarbons, Halogenated; 0/Receptors, Aryl Hydrocarbon; 1746-01-6/Tetrachlorodibenzodioxin; 262-12-4/dibenzo(1,4)dioxin

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