Document Detail

Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules.
MedLine Citation:
PMID:  16815243     Owner:  NLM     Status:  MEDLINE    
The expression of both OAT1 and OAT3 along the isolated rabbit renal proximal tubule (RPT) was determined using RT-PCR. They were found to be very strong in S2 segment and weak in S1 and S3 segments. We further examined the relative transport activity of these transporters in isolated perfused rabbit RPT using [(3)H]para-aminohippurate ([(3)H]PAH), and estrone sulfate ([(3)H]ES) as specific substrates for rbOAT1 and rbOAT3, respectively. The transport activity of OAT1 was in the order S2>S1=S3 segments and that of OAT3 was in the order S1=S2>>S3 segments. The addition of alpha-ketoglutarate (100 muM) in the bathing medium increased both OAT1 and OAT3 transport activities in all segments of proximal tubule. The kinetics of [(3)H]succinic acid transport, used to measure the activity of sodium dicarboxylate transporter 3 (NaDC3), were examined. The J(max) for succinic acid was in the order S2>S3 and unmeasurable in the S1 segment. Our data indicate that both OAT1 and OAT3 play quantitatively significant roles in the renal transport of organic anions along the proximal tubule but predominately in S2 segment. The relative contribution of both transporters depends on their relative expression levels and may possibly be affected by the activity of NaDC3 in RPT.
Anusorn Lungkaphin; Buarong Lewchalermwongse; Varanuj Chatsudthipong
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-20
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1758     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-07-25     Completed Date:  2006-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  789-95     Citation Subset:  IM    
Department of Physiology, Faculty of Medicine, Chiang Mai University, Chaing Mai, Thailand.
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MeSH Terms
Kidney Tubules, Proximal / metabolism*
Organic Anion Transport Protein 1 / genetics,  metabolism*
Organic Anion Transporters, Sodium-Independent / genetics,  metabolism*
Protein Transport
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Succinic Acid / metabolism
Reg. No./Substance:
0/Organic Anion Transport Protein 1; 0/Organic Anion Transporters, Sodium-Independent; 0/RNA, Messenger; 0/organic anion transport protein 3; 110-15-6/Succinic Acid

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