| Relative changes in F-actin during the first cell cycle: evidence for two distinct pools of F-actin in the sea urchin egg. | |
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MedLine Citation:
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PMID: 8860229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cortical actin cytoskeleton undergoes dramatic rearrangements during fertilization of sea urchin eggs. To characterize these changes further, we quantified the relative changes in filamentous actin (F-actin) during fertilization and the first cell cycle in both intact eggs and in isolated cortices by quantitative fluorescence microscopy. The level of F-actin in the intact egg decreased after fertilization and continued to decrease throughout the first cell cycle. By 60 min after fertilization, the level of F-actin had decreased to 50% of the unfertilized sea urchin egg. By cytokinesis, the level of F-actin had decreased to 30% of the unfertilized egg. After completion of cell division, individual blastomeres had 10% of the F-actin in the unfertilized egg. In contrast, there was an increase in cortical F-actin to 370% of the level in the unfertilized egg after fertilization. This increase corresponded to the formation of microvilli. There was little change in the level of cortical F-actin during the first cell cycle. We draw parallels to other systems that increase the amount of F-actin in the Triton-insoluble cytoskeleton by recruiting actin from a Triton-soluble pool of F-actin. |
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Authors:
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R A Heil-Chapdelaine; J J Otto |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Cell motility and the cytoskeleton Volume: 34 ISSN: 0886-1544 ISO Abbreviation: Cell Motil. Cytoskeleton Publication Date: 1996 |
Date Detail:
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Created Date: 1997-05-27 Completed Date: 1997-05-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8605339 Medline TA: Cell Motil Cytoskeleton Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 26-35 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism* Animals Cell Cycle / physiology* Fertilization / physiology* Fluorescent Dyes Microscopy, Fluorescence Neutrophil Activation Ovum* Phalloidine Rhodamines Sea Urchins |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Fluorescent Dyes; 0/Rhodamines; 17466-45-4/Phalloidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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