Document Detail


Relative changes in F-actin during the first cell cycle: evidence for two distinct pools of F-actin in the sea urchin egg.
MedLine Citation:
PMID:  8860229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cortical actin cytoskeleton undergoes dramatic rearrangements during fertilization of sea urchin eggs. To characterize these changes further, we quantified the relative changes in filamentous actin (F-actin) during fertilization and the first cell cycle in both intact eggs and in isolated cortices by quantitative fluorescence microscopy. The level of F-actin in the intact egg decreased after fertilization and continued to decrease throughout the first cell cycle. By 60 min after fertilization, the level of F-actin had decreased to 50% of the unfertilized sea urchin egg. By cytokinesis, the level of F-actin had decreased to 30% of the unfertilized egg. After completion of cell division, individual blastomeres had 10% of the F-actin in the unfertilized egg. In contrast, there was an increase in cortical F-actin to 370% of the level in the unfertilized egg after fertilization. This increase corresponded to the formation of microvilli. There was little change in the level of cortical F-actin during the first cell cycle. We draw parallels to other systems that increase the amount of F-actin in the Triton-insoluble cytoskeleton by recruiting actin from a Triton-soluble pool of F-actin.
Authors:
R A Heil-Chapdelaine; J J Otto
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  34     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  1996  
Date Detail:
Created Date:  1997-05-27     Completed Date:  1997-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  26-35     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Cell Cycle / physiology*
Fertilization / physiology*
Fluorescent Dyes
Microscopy, Fluorescence
Neutrophil Activation
Ovum*
Phalloidine
Rhodamines
Sea Urchins
Chemical
Reg. No./Substance:
0/Actins; 0/Fluorescent Dyes; 0/Rhodamines; 17466-45-4/Phalloidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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