Document Detail

Relative accuracy of the halothane challenge test and a molecular genetic test in detecting the gene for porcine stress syndrome.
MedLine Citation:
PMID:  8392044     Owner:  NLM     Status:  MEDLINE    
Pietrain swine homozygous for the hal gene (n) associated with porcine stress syndrome (PSS) and a Pietrain-derivative breed, Near Pietrain (NP), with a frequency of .31 for n, were mated to produce reciprocal F1, F2, and purebred NP litters. The halothane challenge test was performed on all 40 parents and 240 progeny to predict their hal genotype and PSS susceptibility. The DNA-based assay for a C to T mutation at base pair 1,843 of the skeletal muscle ryanodine receptor (ryr1) cDNA, which is very highly correlated with PSS, was also determined for these animals. The predicted hal genotypes observed in the progeny differed significantly from the expected Mendelian ratios, and the halothane challenge test consistently overestimated the n/n hal genotype. However, the ryr1 genotypes observed in the progeny did not differ significantly from the expected Mendelian ratios, and this DNA-based assay apparently misidentified only one of the 40 parents. The results of this study indicate that the assay for the ryr1 mutation more accurately predicts both the homozygous and heterozygous forms of the PSS gene than does the halothane challenge test.
W E Rempel; M Lu; S el Kandelgy; C F Kennedy; L R Irvin; J R Mickelson; C F Louis
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of animal science     Volume:  71     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-08-10     Completed Date:  1993-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1395-9     Citation Subset:  IM    
Department of Animal Science, University of Minnesota, St. Paul 55108.
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MeSH Terms
Calcium Channels / genetics
DNA / analysis
Disease Susceptibility
Halothane / diagnostic use*
Malignant Hyperthermia / diagnosis,  genetics,  veterinary*
Muscle Proteins / genetics
Predictive Value of Tests
Ryanodine Receptor Calcium Release Channel
Swine Diseases / diagnosis,  genetics*
Reg. No./Substance:
0/Calcium Channels; 0/Muscle Proteins; 0/Ryanodine Receptor Calcium Release Channel; 151-67-7/Halothane; 9007-49-2/DNA

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