Document Detail


Relative ability to provide help: an explanation for Con A-induced suppression.
MedLine Citation:
PMID:  6461448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Concanavalin A (Con A) induced suppression of human peripheral lymphocytes has been studied in vitro at different cell concentrations using a 20 microliters inverted Terasaki plate culture system in which the uptake of 3H-thymidine was measured. Conventional assessment of suppression relates the response of cells to Con A in the presence of Con A-pretreated autologous cells, with the response of cells preincubated without Con A (control cells). The level of suppression so calculated was affected both by serum-related high background counts and by autologous stimulation of responding cells by mitomycin-treated Con A-pretreated cells. The high background was largely removed by the use of serum-free Iscove's medium. The data involving different cell concentrations and periods of cultivation indicate that the assessment of suppression is inadequate. Both Con A-pretreated and control cells are shown to 'help' the response of autologous responder cells to added soluble Con A. When the help by Con A-treated cells is less marked than that by control cells, apparent suppression is seen since the control cell provide the conventional baseline from which suppression is judged. After longer periods of cultivation the Con A-pretreated cells increased responses more than did the control cells leading to apparent enhancement.
Authors:
J Farrant; C Newton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  45     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1981 Sep 
Date Detail:
Created Date:  1982-06-24     Completed Date:  1982-06-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  504-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Concanavalin A / pharmacology*
DNA / biosynthesis
Humans
Leukocyte Count
Lymphocyte Activation / drug effects
Lymphocytes / drug effects,  metabolism*
Mitomycins / pharmacology
T-Lymphocytes / immunology
T-Lymphocytes, Regulatory / immunology
Thymidine / metabolism*
Chemical
Reg. No./Substance:
0/Mitomycins; 11028-71-0/Concanavalin A; 50-89-5/Thymidine; 9007-49-2/DNA
Comments/Corrections

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