| Relative workload determines exercise-induced increases in PGC-1alpha mRNA. | |
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MedLine Citation:
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PMID: 20139785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: The hypothesis that brief intermittent exercise-induced increases in human skeletal muscle metabolic mRNA is dependent on relative workload was investigated. METHODS: Trained (n = 10) and untrained (n = 8) subjects performed exhaustive intermittent cycling exercise (4 x 4 min at 85% of VO(2peak), interspersed by 3 min). Trained subjects also performed the intermittent exercise at the same absolute workload as the untrained subjects, corresponding to 70% of VO(2peak) (n = 6). RESULTS: Exercise at 85% of V(O2peak) elevated (P < 0.001) venous plasma lactate to 10.1 +/- 0.4 and 10.8 +/- 0.5 mM in the trained and untrained subjects, respectively. Peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) mRNA expression was increased (P < 0.001) approximately four- to fivefold for several hours after exercise in both groups. After exercise at 70% of VO(2peak), venous plasma lactate was less (P < 0.001) elevated (3.1 +/- 0.7 mM) and PGC-1alpha mRNA content was less (P < 0.05) increased (approximately threefold) than after exercise at 85% of VO(2peak). Likewise, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA expressions were increased (P < 0.05) only after exercise performed at 85% of VO(2peak) in the trained subjects. Hypoxia-inducible factor 2alpha mRNA only increased (P < 0.05) 3 h into recovery in trained subjects, with no difference between the 70% and 85% of VO(2peak) trial. No change in hypoxia-inducible factor 1alpha, phosphofructokinase, citrate synthase, or lactate dehydrogenase, heart and muscle isoforms, mRNA expressions was detected after any of the exercise trials. CONCLUSIONS: The relative intensity of brief intermittent exercise is of major importance for the exercise-induced increase of several mRNA, including PGC-1alpha. |
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Authors:
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Nikolai Baastrup Nordsborg; Carsten Lundby; Lotte Leick; Henriette Pilegaard |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medicine and science in sports and exercise Volume: 42 ISSN: 1530-0315 ISO Abbreviation: Med Sci Sports Exerc Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-20 Completed Date: 2010-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8005433 Medline TA: Med Sci Sports Exerc Country: United States |
Other Details:
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Languages: eng Pagination: 1477-84 Citation Subset: IM; S |
Affiliation:
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Copenhagen Muscle Research Centre, University of Copenhagen, Denmark. nnordsborg@ifi.ku.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Adult Basic Helix-Loop-Helix Transcription Factors / analysis Exercise / physiology* Exercise Test Heat-Shock Proteins / genetics, metabolism* Hexokinase / analysis Humans Lactic Acid / blood Muscle, Skeletal / chemistry*, physiology* Oxygen Consumption Protein Kinases / analysis RNA, Messenger / metabolism* Transcription Factors / genetics, metabolism* Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Basic Helix-Loop-Helix Transcription Factors; 0/Heat-Shock Proteins; 0/PPARGC1A protein, human; 0/RNA, Messenger; 0/Transcription Factors; 0/endothelial PAS domain-containing protein 1; 50-21-5/Lactic Acid; EC 2.7.-/Protein Kinases; EC 2.7.1.-/pyruvate dehydrogenase kinase 4; EC 2.7.1.1/Hexokinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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