Document Detail


Relationships of plasma and hepatic variables with rates of plasma low-density lipoprotein apolipoprotein B metabolism in baboons fed low- and high-fat diets.
MedLine Citation:
PMID:  7637648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
These studies were conducted to determine relationships of plasma low-density lipoprotein (LDL) cholesterol concentrations and hepatic mRNA levels for apolipoprotein (apo) B, LDL receptor, and hepatic hydroxymethyl glutaryl coenzyme A (HMG CoA) synthase with plasma LDL apo B production and catabolic rates in baboons maintained on a low-cholesterol, low-fat chow diet and on a high-cholesterol, high-fat (HCHF) diet. Twelve baboons with LDL cholesterol levels ranging from low to high on the HCHF diet but with similar high-density lipoprotein (HDL) cholesterol levels were selected from a colony of selectively bred pedigreed baboons. LDL apo B turnover and hepatic mRNA concentrations for apo B, LDL receptor, and HMG CoA synthase were measured on a chow diet and again on a HCHF diet fed for 14 weeks. LDL apo B fractional catabolic rates decreased and production rates increased on the HCHF diet. Hepatic mRNA concentrations for apo B were not affected by the HCHF diet. Hepatic LDL receptor and HMG CoA synthase mRNA concentrations decreased on the HCHF diet as compared with the chow diet. LDL apo B fractional catabolic rate was negatively correlated with plasma cholesterol, LDL cholesterol, LDL apo B, and LDL apo B production and positively correlated with hepatic LDL receptor and HMG CoA synthase mRNA concentrations and with plasma LDL triglyceride to cholesterol ratio on the chow diet but not on the HCHF diet. LDL apo B production was positively correlated with plasma cholesterol, LDL cholesterol, and LDL apo B on the HCHF diet and negatively correlated with LDL triglyceride to cholesterol ratio on both chow and HCHF diets.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
R S Kushwaha; P H Barrett; C A Reardon; D S Lewis; K D Carey; G S Getz; H C McGill
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  44     ISSN:  0026-0495     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-12     Completed Date:  1995-09-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1058-66     Citation Subset:  IM    
Affiliation:
Department of Physiology and Medicine, Southwest Foundation for Biomedical Research, San Antonio, TX 78228-0147, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins B / blood*,  genetics,  metabolism
Cholesterol, Dietary / administration & dosage
Cholesterol, LDL / blood
Diet, Fat-Restricted / veterinary*
Dietary Fats / administration & dosage*
Female
Hydroxymethylglutaryl-CoA Synthase / analysis,  genetics,  physiology
Lipoproteins, LDL / blood*,  metabolism
Liver / chemistry,  enzymology
Male
Papio / metabolism*
RNA, Messenger / analysis,  genetics
Receptors, LDL / analysis,  genetics
Triglycerides / blood
Grant Support
ID/Acronym/Agency:
HL28972/HL/NHLBI NIH HHS; HL41256/HL/NHLBI NIH HHS; RR02176/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins B; 0/Cholesterol, Dietary; 0/Cholesterol, LDL; 0/Dietary Fats; 0/Lipoproteins, LDL; 0/RNA, Messenger; 0/Receptors, LDL; 0/Triglycerides; EC 2.3.3.10/Hydroxymethylglutaryl-CoA Synthase

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