Document Detail


Relationships between fiber composition and NMR measurements in human skeletal muscle.
MedLine Citation:
PMID:  8842027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to examine the relationships between the relative contents of phosphocreatine (PCr), inorganic phosphate (Pi), beta-adenosine triphosphate (ATP), and transverse relaxation time (T2) with fiber composition, which determined histochemically in the human skeletal muscle. The vastus lateralis muscles of 28 volunteers were subjected to phosphorus nuclear magnetic resonance (31P NMR) spectroscopy, magnetic resonance imaging (MRI) and muscle biopsy. Muscle fibers were divided into type I and type II fibers using myosin ATPase stain. A wide range of fiber composition levels were observed in the subjects (27.3-74.6% type I fibers). The PCr/ATP, Pi/ATP and (PCr + Pi)/ATP ratios were positively related to the percentage of type II fibers (r = 0.695, p < 0.001, r = 0.429, p < 0.05 and r = 0.773, p < 0.001, respectively). There was no correlation between fiber composition and the PCr/Pi ratio (r 0.127, n.s.) or intracellular pH (r = 0.305, n.s.). Moreover, no correlation was found between T2 and fiber type (r = 0.144, n.s.). These results suggest that 31P NMR can detect the differences in relative content of phosphates between type I and type II fibers, thereby noninvasively evaluating fiber composition in human skeletal muscle.
Authors:
H Takahashi; S Y Kuno; S Katsuta; H Shimojo; K Masuda; H Yoshioka; I Anno; Y Itai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  NMR in biomedicine     Volume:  9     ISSN:  0952-3480     ISO Abbreviation:  NMR Biomed     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-12-19     Completed Date:  1996-12-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8915233     Medline TA:  NMR Biomed     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  8-12     Citation Subset:  IM    
Affiliation:
Department of Radiology, University of Tsukuba, Ibaraki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analysis
Adolescent
Adult
Biopsy, Needle
Female
Histocytochemistry
Humans
Magnetic Resonance Imaging
Magnetic Resonance Spectroscopy*
Male
Muscle Fibers, Skeletal / chemistry*,  classification
Muscle Relaxation
Muscle, Skeletal / chemistry*,  physiology
Phosphates / analysis
Phosphocreatine / analysis
Chemical
Reg. No./Substance:
0/Phosphates; 56-65-5/Adenosine Triphosphate; 67-07-2/Phosphocreatine

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