Document Detail


Relationships between colony forming efficiency and parameters of intrinsic radiosensitivity.
MedLine Citation:
PMID:  10549604     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: In an attempt to determine whether radiosensitivity is correlated with colony forming efficiency (CFE), a large amount of data have been analysed from the literature. MATERIALS AND METHODS: The survival curves of 446 human cell lines irradiated in exponentially growing phase in vitro are included in this study. Technical factors such as culture type and the use of feeder cells were considered cofactors in addition to the genetic and histological origin of the cells. Intrinsic radiosensitivity is expressed in terms of the parameters of the linear quadratic model and the single-hit multitarget model. RESULTS: It is shown that low CFE is characteristic of cells plated in agar and cells from primary biopsies. Cells plated in the presence of feeder cells have, in general, higher CFE than cells plated without feeder cells. A positive correlation is observed between intrinsic radiosensitivity and CFE: the higher the CFE, the more resistant the cell line. This relationship is particularly obvious when radiosensitivity is expressed in terms of alpha, S2 or D, parameters which essentially characterize the initial part of the survival curve. The correlation is also found within histological or genetic groups of cell lines. However, for a given cell line, there is no relationship between CFE and radiosensitivity among different experiments. Cells irradiated in the presence of feeder cells are less subject to this behaviour. CONCLUSIONS: CFE as well as radiosensitivity are intrinsic properties of a cell line. Experimental conditions determine the quality of the correlation between radiosensitivity and CFE.
Authors:
B Fertil; P J Deschavanne
Related Documents :
1438704 - Low ph does not affect the dose response for 5'-amino-5'-deoxythymidine modulation of i...
16819144 - Protective effects of melatonin against low- and high-let irradiation.
212214 - Application of alkaline sucrose gradient sedimentation to the study of dna damage and i...
1969914 - Mechanisms of cell reproductive death and shapes of radiation dose--survival curves of ...
25314674 - Toward practical application of functional conductive polymer binder for a high-energy ...
1313814 - Nerve growth factor nonresponsive pheochromocytoma cells: altered internalization resul...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of radiation biology     Volume:  75     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-15     Completed Date:  1999-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1275-82     Citation Subset:  IM; S    
Affiliation:
INSERM U.494, 91 bl de l'Hôpital, Paris, France. fertil@imed.jussieu.fr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Survival / radiation effects
Clone Cells / radiation effects
Colony-Forming Units Assay*
Humans
Models, Biological
Radiation Tolerance*
Radiobiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Influence of dose-rate, post-irradiation incubation time and growth factors on interphase cell death...
Next Document:  Modulation of the clastogenic activity of gamma-irradiation in buthionine sulphoximine-mediated glut...