Document Detail


Relationships between age and epi-genotype of the FMR1 exon 1/intron 1 boundary are consistent with non-random X-chromosome inactivation in FM individuals, with the selection for the unmethylated state being most significant between birth and puberty.
MedLine Citation:
PMID:  23307923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methylation of the fragile X-related epigenetic element 2 (FREE2) located on the exon 1/intron 1 boundary of the FMR1 gene is related to FMRP expression and cognitive impairment in full mutation (FM; CGG>200) individuals. We examined the relationship between age, the size of the FMR1 CGG expansion and the methylation output ratio (MOR) at 12 CpG sites proximal to the exon 1/intron 1 boundary using FREE2 MALDI-TOF MS. The patient cohort included 119 males and 368 females, i.e. 121 healthy controls (CGG<40), 176 premutation (CGG 55-170) and 190 FM (CGG 213-2000). For all CpG units examined, FM males showed a significantly elevated MOR compared with that in hypermethylated FM females. In FM males the MOR for most CpG units significantly positively correlated with both age and CGG size (P< 0.05). In FM females the skewing towards the unmethylated state was significant for half of the units between birth and puberty (P < 0.05). The methylation status of intron 1 CpG10-12 that was most significantly related to cognitive impairment in our earlier study, did not change significantly with age in FM females. These results challenge the concept of fragile X syndrome (FXS)-related methylation being static over time, and suggest that due to the preference for the unmethylated state in FM females, X-inactivation at this locus is not random. The findings also highlight that the prognostic value of FXS methylation testing is not uniform between all CpG sites, and thus may need to be evaluated on a site-by-site basis.
Authors:
David E Godler; Yoshimi Inaba; Elva Z Shi; Cindy Skinner; Quang M Bui; David Francis; David J Amor; John L Hopper; Danuta Z Loesch; Randi J Hagerman; Charles E Schwartz; Howard R Slater
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-10
Journal Detail:
Title:  Human molecular genetics     Volume:  22     ISSN:  1460-2083     ISO Abbreviation:  Hum. Mol. Genet.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-25     Completed Date:  2013-09-03     Revised Date:  2014-04-15    
Medline Journal Info:
Nlm Unique ID:  9208958     Medline TA:  Hum Mol Genet     Country:  England    
Other Details:
Languages:  eng     Pagination:  1516-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Age Factors
Aged
Aged, 80 and over
Child
Child, Preschool
CpG Islands / genetics
Epigenesis, Genetic / genetics*
Exons
Female
Fragile X Mental Retardation Protein / genetics*
Fragile X Syndrome / genetics*
Humans
Infant
Infant, Newborn
Male
Methylation
Middle Aged
Mutation / genetics
RNA-Binding Proteins / genetics*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
X Chromosome Inactivation / genetics*
Grant Support
ID/Acronym/Agency:
HD36071/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/FMR1 protein, human; 0/FXR2 protein, human; 0/RNA-Binding Proteins; 139135-51-6/Fragile X Mental Retardation Protein
Comments/Corrections

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