Document Detail


Relationships between levels of urinary podocalyxin, number of urinary podocytes, and histologic injury in adult patients with IgA nephropathy.
MedLine Citation:
PMID:  22700887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Podocalyxin (PCX) is present on the apical cell membrane of podocytes and is shed in urine from injured podocytes. Urinary podocalyxin (u-PCX) is associated with severity of active glomerular injury in patients with glomerular diseases. This study examined the relationship between number of urinary podocytes, levels of u-PCX, and glomerular injury in adults with IgA nephropathy (IgAN).
DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Urine samples voided in the morning on the day of biopsy were obtained from 51 patients with IgAN (18 men and 33 women; mean age, 31 years). All renal biopsy specimens were analyzed histologically. Pathologic variables of IgAN were analyzed per Shigematsu classification, the Oxford classification of IgAN, and the Clinical Guidelines of IgAN in Japan. Levels of u-PCX were measured by sandwich ELISA.
RESULTS: Histologic analysis based on Shigematsu classification revealed a significant correlation between levels of u-PCX and severity of acute extracapillary abnormalities (r=0.72; P<0.001), but levels of urinary protein excretion did not correlate with acute glomerular abnormalities. Levels of urinary protein excretion in patients with segmental sclerosis (n=19) were higher than in patients without (n=22) (0.49 [interquartile range (IQR), 0.20-0.88] g/g creatinine versus 0.20 [IQR, 0.10-0.33] g/g creatinine; P<0.01). The number of urinary podocytes in patients with segmental sclerosis was higher than in patients without (1.05 [IQR, 0.41-1.67] per mg creatinine versus 0.28 [IQR, 0.10-0.66] per mg creatinine; P<0.01).
CONCLUSIONS: Levels of u-PCX and the number of urinary podocytes are associated with histologic abnormalities in adults with IgAN.
Authors:
Rin Asao; Katsuhiko Asanuma; Fumiko Kodama; Miyuki Akiba-Takagi; Yoshiko Nagai-Hosoe; Takuto Seki; Yukihiko Takeda; Isao Ohsawa; Satoshi Mano; Kiyoshi Matsuoka; Hiroyuki Kurosawa; Shinya Ogasawara; Yoshiaki Hirayama; Sakari Sekine; Satoshi Horikoshi; Masanori Hara; Yasuhiko Tomino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-14
Journal Detail:
Title:  Clinical journal of the American Society of Nephrology : CJASN     Volume:  7     ISSN:  1555-905X     ISO Abbreviation:  Clin J Am Soc Nephrol     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-10     Completed Date:  2013-01-29     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  101271570     Medline TA:  Clin J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1385-93     Citation Subset:  IM    
Affiliation:
Division of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / blood,  urine
Biopsy
Creatinine / blood,  urine
Disease Progression
Enzyme-Linked Immunosorbent Assay
Female
Glomerulonephritis, IGA / blood,  complications,  diagnosis*,  pathology,  urine
Glomerulosclerosis, Focal Segmental / diagnosis*,  etiology,  pathology,  urine
Humans
Japan
Male
Podocytes / metabolism*,  pathology*
Predictive Value of Tests
Proteinuria / diagnosis,  etiology,  urine
Sensitivity and Specificity
Severity of Illness Index
Sialoglycoproteins / urine*
Urine / cytology
Young Adult
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Sialoglycoproteins; 0/podocalyxin; 60-27-5/Creatinine

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