| Relationships between uterine blood flow, peripheral sex steroids, expression of endometrial estrogen receptors and nitric oxide synthases during the estrous cycle in mares. | |
| | |
MedLine Citation:
|
PMID: 20953124 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The objective of this study was to investigate the relationships between uterine perfusion and estrogen, progesterone and the uterine nitric oxide synthase (NOS) system in five trotter mares during the estrous cycle. Color Doppler sonography for measurement of uterine blood flow and collection of blood for determination of plasma estrogen and progesterone concentrations were performed on days 0 (= ovulation), 1, 5, 11 and 15 and daily during estrus (days -1 to -4) of one estrous cycle; endometrial biopsy collection for mRNA expression analysis of NOS and estrogen receptors was performed on days 0, 1, 5, 11, 15 and -3. Blood flow in each uterine artery was assessed by calculating the mean time-averaged maximum velocity (TAMV) and the pulsatility index (PI). Plasma concentrations of estrogen and progesterone were determined using specific enzyme immunoassays. The mRNA expressions of endothelial NOS (eNOS), inducible NOS (iNOS) as well as estrogen receptors α (ERα) and β (ERβ) were quantified using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. The TAMV and PI had a biphasic pattern during the estrous cycle (P<0.05), with maximum and minimum, respectively, values on days 5 and -4. Estrogen receptor mRNA concentrations increased significantly during days 15 (ERα) and -3 (ERβ). Transcript expression of eNOS, but not iNOS, had a biphasic pattern during the cycle (P<0.05) with maximum levels on days 5 and -3 and correlated positively with TAMV (r=0.81, P=0.05). We infer that the uterine NOS system, especially eNOS, plays an important role in the regulation of uterine blood flow during the estrous cycle in mares. |
| | |
Authors:
|
Aenne Honnens; Simone Weisser; Harald Welter; Ralf Einspanier; Heinrich Bollwein |
Related Documents
:
|
1592284 - New pelvic sonoangiography for detection of endometrial carcinoma: a preliminary report. 8743144 - Interrelationships between the renin angiotensin system and uteroplacental blood flow--... 3706444 - Maternal ovine placental vascular responses to adenosine. 3987274 - Ethynyl-estradiol (ee-2) retained in blood and endometrium during and after steroidal c... 15668954 - Peripheral blood mds score: a new flow cytometric tool for the diagnosis of myelodyspla... 6818134 - Abnormal blood group galactosyltransferase in blood type a1b-subjects whose sera contai... |
Publication Detail:
|
Type: Journal Article Date: 2010-10-12 |
Journal Detail:
|
Title: The Journal of reproduction and development Volume: 57 ISSN: 1348-4400 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-03-22 Completed Date: 2011-08-04 Revised Date: 2011-12-13 |
Medline Journal Info:
|
Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan |
Other Details:
|
Languages: eng Pagination: 43-8 Citation Subset: IM |
Affiliation:
|
Clinic for Cattle, School of Veterinary Medicine Hannover, 30173 Hannover, Germany. aenne.honnens@tiho-hannover.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biopsy / veterinary Blood Flow Velocity / veterinary Endometrium / cytology, metabolism* Estrogens / blood Estrous Cycle / blood*, physiology* Female Gene Expression Regulation Gonadal Steroid Hormones / blood Horses / physiology* Nitric Oxide Synthase / genetics, metabolism* Progesterone / blood Protein Isoforms / genetics, metabolism RNA, Messenger / metabolism Receptors, Estrogen / genetics, metabolism* Regional Blood Flow Reverse Transcriptase Polymerase Chain Reaction / veterinary Ultrasonography, Doppler, Color / veterinary Uterine Artery / ultrasonography Uterus / blood supply*, ultrasonography |
| Chemical | |
Reg. No./Substance:
|
0/Estrogens; 0/Gonadal Steroid Hormones; 0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Estrogen; 57-83-0/Progesterone; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without a...
Next Document: Usage of analgesic in a murine model infected latently with pseudorabies virus.