Document Detail

Relationship of nutritional factors to in vitro tumor cell growth and cytotoxicity produced by cytosine arabinoside.
MedLine Citation:
PMID:  558053     Owner:  NLM     Status:  MEDLINE    
The in vitro relationship between nutritional factors, proliferative status of tumor cells, and the cytotoxic action of cytosine arabinoside (ara-C) was investigated. The reduction in the concentration of only one essential amino acid, L-isoleucine, in the growth medium of A(T1)C1-3 hamster fibrosarcoma cells decreased DNA synthesis in this cell population and slowed the rate of progression of G1 phase cells into S phase of the cell cycle. The complete omission of isoleucine from the growth medium blocked the progression of G1 phase cells into S phase and prevented the cytotoxic action of ara-C. The addition of isoleucine to the isoleucine-deprived cells permitted these cells to enter the S phase and restored their sensitivity to the cytotoxic action of ara-C. When G1 phase cells were placed in a medium containing reduced levels of all the amino acids and vitamins there was a prolongation of the G1 phase. Since medium with low levels of amino acids produced a delay in the entry of G1 phase cells into the S phase, the time interval in which these cells were most sensitive to the cytotoxic action of ara-C was different for G1 phase cells placed in medium with adequate levels of all the amino acids. These in vitro data indicate that nutritional factors can markedly effect the proliferation of tumor cells and the cytotoxic action of ara-C.
R L Momparler; J Goodman
Related Documents :
7510743 - Sensitivity of human leukemia cells in exponential or stationary growth phase to anti-c...
8390033 - Regulation of muscarinic stimulation of norepinephrine release and pi hydrolysis during...
24194143 - Testicular responsiveness to gonadotropic hormonein vitro and leydig and sertoli cell u...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  10     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-06-11     Completed Date:  1977-06-11     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  127-35     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Division / drug effects
Cell Line
Cytarabine / pharmacology*
DNA, Neoplasm / biosynthesis*
Fibrosarcoma* / metabolism,  pathology
Isoleucine / metabolism*
Reg. No./Substance:
0/DNA, Neoplasm; 147-94-4/Cytarabine; 73-32-5/Isoleucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cell density dependence of focus formation in the C3H/10T1/2 transformation assay.
Next Document:  The kinetics of contact inhibition in mammalian cells.