| Relationship of non-esterified fatty acids to vitamin D-dependent Ca2+ binding by rat intestinal Golgi-enriched membrane fractions. | |
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MedLine Citation:
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PMID: 6712618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ca2+ binding and concentrations of non-esterified fatty acids and phospholipids were compared in membrane fractions of rat small intestine. These fractions differed in density and were enriched for galactosyltransferase activity, a Golgi-membrane marker. Ca2+ binding was highest in the Golgi subfraction with the least density, as were the concentrations of both non-esterified fatty acids and phospholipids; galactosyltransferase activity was distributed differently. The large amount of non-esterified fatty acids was sufficient to account for a 2:1 complex of fatty acid-Ca2+. In vitamin D-deficient animals, the yield of protein in the lightest subfractions was decreased, but Ca2+ binding per mg of protein was further decreased to about 60%. In Golgi fractions from vitamin D-deficient animals, Ca2+ binding and the concentration of non-esterified fatty acids were decreased in parallel, but phospholipids were not significantly changed. There was a close correlation between Golgi Ca2+ binding and non-esterified fatty acid concentrations (r = 0.89; P less than 0.001). Non-esterified fatty acids, which are unusually prevalent in these membrane fractions, are likely to be the binding sites that account for this vitamin D-dependent Ca2+ uptake. |
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Authors:
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J R Walters; M M Weiser |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Biochemical journal Volume: 218 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1984 Mar |
Date Detail:
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Created Date: 1984-05-10 Completed Date: 1984-05-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 355-60 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Calcium / metabolism* Cell Membrane / drug effects, metabolism Fatty Acids, Nonesterified / metabolism* Golgi Apparatus / drug effects, metabolism* Intestine, Small / drug effects, metabolism* Male Phospholipids / metabolism Rats Vitamin D / pharmacology* Vitamin D Deficiency / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AM07336/AM/NIADDK NIH HHS; AM25754/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Phospholipids; 1406-16-2/Vitamin D; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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