Document Detail

Relationship between visualization of meiotic spindle in human oocytes and ICSI outcomes: a meta-analysis.
MedLine Citation:
PMID:  19192344     Owner:  NLM     Status:  MEDLINE    
The objective of this meta-analysis was to investigate the influence of meiotic spindle visualization in human oocytes on intracytoplasmic sperm injection (ICSI) outcomes. Search strategies included on-line surveys of databases (MEDLINE, EMBASE, Science Citation Index, Cochrane Controlled Trials Register and Ovid). The fixed effect was used for odds ratio. Ten trials fulfilled the inclusion criteria comparing in-vitro and clinical ICSI outcomes with or without visualization of meiotic spindle in fresh and in-vivo matured oocytes. According to the meta-analysis, the results showed statistically significant higher fertilization rate (P < 0.0001) when the meiotic spindle was viewed than when it was not. Moreover, the percentage of pro-nuclear-stage embryos with good morphology (P = 0.003), cleavage rate (P < 0.0001), percentage of day-3 top-quality embryos (P = 0.003) and percentage of embryos that reached the blastocyst stage (P < 0.0001) were statistically significantly better among embryos derived from oocytes in which meiotic spindle was viewed compared with those in which meiotic spindle was not observed. However, these differences were not observed in the clinical pregnancy or implantation rates. This observation has clinical relevance mainly in countries where there is a legal limit on the number of oocytes to be fertilized. However, additional controlled trials are needed to further confirm these results.
C G Petersen; J B A Oliveira; A L Mauri; F C Massaro; R L R Baruffi; A Pontes; J G Franco
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Publication Detail:
Type:  Journal Article; Meta-Analysis    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  18     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-04     Completed Date:  2009-05-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  235-43     Citation Subset:  IM    
Center for Human Reproduction Prof. Franco Junior, Av. Prof. João Fiusa, 689, 14025-310, Ribeirão Preto, SP, Brazil.
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MeSH Terms
Cleavage Stage, Ovum / physiology
Cytological Techniques
Embryonic Development / physiology
Infertility / diagnosis
Mitotic Spindle Apparatus / physiology,  ultrastructure*
Oocytes / cytology,  ultrastructure*
Pregnancy Rate
Sperm Injections, Intracytoplasmic* / methods
Treatment Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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