Document Detail

Relationship between tumour oxygenation, bioenergetic status and radiobiological hypoxia in an experimental model.
MedLine Citation:
PMID:  7779418     Owner:  NLM     Status:  MEDLINE    
Tumour oxygenation and bioenergetic status were measured in the same tumour and these results related to radiobiological hypoxia. A C3H mouse mammary carcinoma grown in the feet of CDF1 mice was used. Bioenergetic status was assessed by 31P MRS using a SISCO 7 Tesla magnet, oxygen measurements were done by a polarographic electrode and the hypoxic fraction was determined from direct analysis of the radiation dose-response data. During all examinations restrained, non-anaesthetized mice were allowed to breathe either 100% oxygen, carbogen, normal air, carbon monoxide (CO) at 75, 220, or 660 ppm or had blood flow occluded by clamping. Results showed a significant correlation between the radiobiological hypoxic fraction and % pO2 < or = 5 mmHg under the different treatment conditions, whereas no correlation was found between beta nucleosidetriphosphate/inorganic phosphate (beta-NTP/Pi) ratio and either the hypoxic fraction or the % of pO2 values < or = 5 mmHg under the different treatment conditions. In conclusion, oxygen electrode measurements were sensitive to changes in tumour hypoxia whereas the bioenergetic status alone seemed to be a less precise measure of hypoxia in this tumour model. Furthermore, the present study demonstrated that tumour cells in vivo can actually maintain the bioenergetic status during a period of severe hypoxia.
M Nordsmark; C Grau; M R Horsman; H S Jörgensen; J Overgaard
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta oncologica (Stockholm, Sweden)     Volume:  34     ISSN:  0284-186X     ISO Abbreviation:  Acta Oncol     Publication Date:  1995  
Date Detail:
Created Date:  1995-07-18     Completed Date:  1995-07-18     Revised Date:  2009-05-12    
Medline Journal Info:
Nlm Unique ID:  8709065     Medline TA:  Acta Oncol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  329-34     Citation Subset:  IM    
Danish Cancer Society, Department of Experimental Clinical Oncology, Aarhus.
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MeSH Terms
Carbon Dioxide / pharmacology
Cell Hypoxia
Energy Metabolism* / drug effects
Magnetic Resonance Spectroscopy / methods
Mammary Neoplasms, Experimental / blood supply,  metabolism*
Mice, Inbred C3H
Mice, Inbred DBA
Oxygen / analysis,  pharmacology
Oxygen Consumption* / drug effects
Partial Pressure
Phosphates / metabolism
Radiation-Sensitizing Agents / pharmacology
Ribonucleotides / metabolism
Reg. No./Substance:
0/Phosphates; 0/Radiation-Sensitizing Agents; 0/Ribonucleotides; 124-38-9/Carbon Dioxide; 7723-14-0/Phosphorus; 7782-44-7/Oxygen; 8063-77-2/carbogen

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