Document Detail


Relationship between seminal antioxidant enzymes and the phospholipid and fatty acid composition of spermatozoa.
MedLine Citation:
PMID:  18492368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sperm cell membranes are susceptible to peroxidative damage through an excess of reactive oxygen species. The objective of this study was to determine seminal plasma glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity and relate these to phospholipid profiles and phospholipid-esterified fatty acid composition of spermatozoa. Seminal plasma GPX and SOD activity, phospholipid, phospholipid-esterified fatty acid composition and malondialdehyde (MDA) of spermatozoa were assayed in 10 normozoospermic and 25 asthenozoospermic subjects. Mean seminal GPX and SOD activity in normozoospermic men were not significantly different from asthenozoospermic men. A significant positive correlation was observed between seminal plasma GPX activity and phosphatidylcholine content (r = +0.77, P = 0.037) and there was a significant negative correlation with lysophosphatidylcholine content (r = -0.89, P = 0.02) in normozoospermic sperm samples. Positive correlations were found between SOD activity and polyunsaturated fatty acid composition of spermatozoa. MDA content in the spermatozoa of asthenozoospermic subjects was significantly higher than in normozoospermic males (P < 0.05). Negative correlations were found between MDA content and seminal SOD activity and arachidonic acid content of spermatozoa from normozoospermic samples (r = -0.5; P = 0.046, r = -0.9; P = 0.001 respectively). Seminal plasma GPX and SOD provide protection against lipid peroxidation of phospholipid and phospholipid-bound fatty acids in normozoospermic samples.
Authors:
Heidar Tavilani; Mohamad Taghi Goodarzi; Mahmoud Doosti; Asad Vaisi-Raygani; Taghi Hassanzadeh; Saeedeh Salimi; Hamid Reza Joshaghani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  16     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-21     Completed Date:  2008-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  649-56     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran. tavilani@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / metabolism*
Fatty Acids / metabolism*
Glutathione Peroxidase / metabolism*
Humans
Male
Malondialdehyde / metabolism
Phospholipids / metabolism*
Semen / enzymology*
Spermatozoa / metabolism*
Superoxide Dismutase / metabolism*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Fatty Acids; 0/Phospholipids; 542-78-9/Malondialdehyde; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

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