Document Detail

Relationship between protein synthesis and viral deoxyribonucleic acid synthesis.
MedLine Citation:
PMID:  4248262     Owner:  NLM     Status:  MEDLINE    
Evidence is presented that poxvirus deoxyribonucleic acid (DNA) synthesis required concurrent protein synthesis. The protein requirement in question can be distinguished from viral-induced thymidine kinase and DNA polymerase by virture of the instability of its messenger ribonucleic acid and its stoichiometric rather than catalytic relation to DNA synthesis. The protein(s) required did accumulate in the presence of fluorodeoxyuridine, an inhibitor of DNA synthesis, and, thus, appeared to be an "early" poxvirus function. The protein(s) was stable since it did function several hours after its synthesis had been terminated by puromycin. Two possible roles for such a protein requirement are discussed.
J R Kates; B R McAuslan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  1     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1967 Feb 
Date Detail:
Created Date:  1970-11-06     Completed Date:  1970-11-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  110-4     Citation Subset:  IM    
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MeSH Terms
Culture Techniques
Cycloheximide / pharmacology
DNA Nucleotidyltransferases
DNA Replication*
DNA, Viral / biosynthesis*
Dactinomycin / pharmacology
Floxuridine / pharmacology
Hela Cells*
Puromycin / pharmacology
RNA, Viral / biosynthesis*
Thymidine Kinase
Uridine / pharmacology
Viral Proteins / biosynthesis*
Virus Cultivation*
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral; 0/Viral Proteins; 10028-17-8/Tritium; 50-76-0/Dactinomycin; 50-89-5/Thymidine; 50-91-9/Floxuridine; 53-79-2/Puromycin; 58-96-8/Uridine; 66-81-9/Cycloheximide; EC Kinase; EC 2.7.7.-/DNA Nucleotidyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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