Document Detail


Relationship between protein phosphatase type-2C activity and induction of apoptosis in cultured neuronal cells.
MedLine Citation:
PMID:  12106776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cellular composition and concentration of fatty acids are crucial for proliferation and survival. We recently showed stimulation of protein phosphatase type-2C (PP2C) by unsaturated fatty acids. Here, we describe that treatment of cultured chick neurons with 100 microM oleic acid for 24h increased the percentage of damaged neurons to 61+/-9% compared with 25+/-4% in controls. Oleic acid-induced cell death showed features of apoptosis such as chromatin condensation, shrinkage of the nucleus, DNA fragmentation and caspase-3 activation. Extensive studies with a variety of fatty acids revealed a striking correlation between activation of PP2C and induction of apoptosis. Lipophilicity, oxidizability, and an acidic group were required for both effects. In addition, activation of PP2C and induction of apoptosis could discriminate between cis- and trans-conformation of the fatty acids. The results are in favor of PP2C playing an important, yet unidentified role in apoptosis.
Authors:
Susanne Klumpp; Dagmar Selke; Barbara Ahlemeyer; Christine Schaper; Josef Krieglstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemistry international     Volume:  41     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-07-10     Completed Date:  2002-10-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  251-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Pharmacy, Philipps-University, Marburg, Germany. klumpp@mailer.uni-marburg.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis* / drug effects
Cells, Cultured
Chick Embryo
Enzyme Activation
Neurons / cytology,  enzymology*
Oleic Acid / pharmacology
Phosphoprotein Phosphatases / metabolism*
Protein Phosphatase 2
Saccharomyces cerevisiae Proteins*
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 112-80-1/Oleic Acid; EC 3.1.3.16/PTC1 protein, S cerevisiae; EC 3.1.3.16/Phosphoprotein Phosphatases; EC 3.1.3.16/Protein Phosphatase 2; EC 3.1.3.16/protein phosphatase 2C

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