| Relationship between plasma free fatty acid, intramyocellular triglycerides and long-chain acylcarnitines in resting humans. | |
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MedLine Citation:
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PMID: 19858228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We hypothesized that plasma non-esterified fatty acids (NEFA) are trafficked directly to intramyocellular long-chain acylcarnitines (imLCAC) rather than transiting intramyocellular triglycerides (imTG) on the way to resting muscle fatty acid oxidation. Overnight fasted adults (n = 61) received intravenous infusions of [U-(13)C]palmitate (0400-0830 h) and [U-(13)C]oleate (0800-1400 h) labelling plasma NEFA, imTG, imLCAC and im-non-esterified FA (imNEFA). Two muscle biopsies (0830 and 1400 h) were performed following 6 h, overlapping, sequential palmitate/oleate tracer infusions. Enrichment of plasma palmitate was approximately 15 times greater than enrichment of imTG, imNEFA-palmitate and im-palmitoyl-carnitine. Fatty acid enrichment in LCAC was correlated with imTG and imNEFA; there was a significant correlation between imTG concentrations and imLCAC concentrations in women (r = 0.51, P = 0.005), but not men (r = 0.30, P = 0.11). We estimated that approximately 11% of NEFA were stored in imTG. imTG NEFA storage was correlated only with NEFA concentrations (r = 0.52, P = 0.004) in women and with V(O(2),peak) (r = 0.45, P = 0.02) in men. At rest, plasma NEFA are trafficked largely to imTG before they enter LCAC oxidative pools; thus, imTG are an important, central pool that regulates the delivery of fatty acids to the intracellular environment. Factors relating to plasma NEFA storage into imTG differ in men and women. |
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Authors:
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Jill A Kanaley; Samyah Shadid; Michael T Sheehan; ZengKui Guo; Michael D Jensen |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of physiology Volume: 587 ISSN: 1469-7793 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-02-26 Revised Date: 2010-12-16 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 5939-50 Citation Subset: IM |
Affiliation:
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Mayo Clinic, Endocrine Research Unit, 200 1st Street SW, Rm 5-194 Joseph, Rochester, MN 55905, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Carnitine / analogs & derivatives*, blood Fatty Acids, Nonesterified / blood* Female Humans Male Muscle Fibers, Skeletal / metabolism* Rest / physiology* Triglycerides / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK40484/DK/NIDDK NIH HHS; DK50456/DK/NIDDK NIH HHS; R37 DK040484-22/DK/NIDDK NIH HHS; RR00585/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Triglycerides; 0/acylcarnitine; 541-15-1/Carnitine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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