Document Detail

Relationship between physical activity level, telomere length, and telomerase activity.
MedLine Citation:
PMID:  18799986     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The purpose of this study was to examine the relationship of exercise energy expenditure (EEE) with both telomere length and telomerase activity in addition to accounting for hTERT C-1327T promoter genotype.
METHODS: Sixty-nine (n = 34 males; n = 35 females) participants 50-70 yr were assessed for weekly EEE level using the Yale Physical Activity Survey. Lifetime consistency of EEE was also determined. Subjects were recruited across a large range of EEE levels and separated into quartiles: 0-990, 991-2340, 2341-3540, and >3541 kcal x wk(-1). Relative telomere length and telomerase activity were measured in peripheral blood mononuclear cells (PBMC).
RESULTS: The second EEE quartile exhibited significantly longer telomere lengths [1.12 +/- 0.03 relative units (RU)] than both the first and fourth EEE quartiles (0.94 +/- 0.03 and 0.96 +/- 0.03 RU, respectively; P < 0.05) but was not different from the third quartile. Telomerase activity was not different among the EEE quartiles. An association was observed between telomerase enzyme activity and hTERT genotype with the TT genotype (1.0 x 10(-2) +/- 4.0 x 10(-3) attomoles (amol) per 10,000 cells; n = 19) having significantly greater telomerase enzyme activity than both the CT (1.3 x 10(-3) +/- 3.2 x 10(-3); n = 30) and CC groups (5.0 x 10(-4) +/- 3.9 x 10(-3); n = 20; P = 0.01).
CONCLUSION: These results indicate that moderate physical activity levels may provide a protective effect on PBMC telomere length compared with both low and high EEE levels.
Andrew T Ludlow; Jo B Zimmerman; Sarah Witkowski; Joe W Hearn; Bradley D Hatfield; Stephen M Roth
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  40     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-18     Completed Date:  2008-12-30     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1764-71     Citation Subset:  IM; S    
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MeSH Terms
Base Sequence
DNA Primers
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
Stress, Psychological
Telomerase / genetics,  metabolism*
Grant Support
AG022791/AG/NIA NIH HHS; AG025505/AG/NIA NIH HHS; K01 AG022791/AG/NIA NIH HHS; K01 AG022791-05/AG/NIA NIH HHS; L30 AG024705/AG/NIA NIH HHS; L30 AG024705-02/AG/NIA NIH HHS; L30 AG024705-03/AG/NIA NIH HHS; R21 AG025505-02/AG/NIA NIH HHS
Reg. No./Substance:
0/DNA Primers; EC protein, human; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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