| Relationship between metabolism and biomass of medically important zygomycetes. | |
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MedLine Citation:
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PMID: 16882609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Little is known about the relationships between metabolic activity and fungal biomass or time of incubation for medically important fungal pathogens. Understanding these relationships may be especially relevant for rapidly growing organisms, such as zygomycetes. A range of inocula of five clinical isolates of zygomycetes (one each of Rhizopus oryzae,Rhizopus microsporus, Cunninghamella bertholletiae, Mucor circinelloides and Absidia corymbifera) were incubated for 6, 8, 12, 24 and 48 h, after which hyphal mass was assessed spectrophotometrically and metabolic activity was measured using various concentrations of XTT and menadione. Both linear regression and the Boltzmann sigmoid model were used and compared for description of relationships between metabolic activity, biomass and time of incubation. Modeling was further applied to eleven additional zygomycete isolates. The relationships of biomass or metabolic activity as a function of time of incubation were well described with the Boltzmann sigmoid model. The latter was superior to linear regression in describing the relationship between metabolic activity and fungal biomass. For all isolates of zygomycetes, increases in metabolic activity preceded increases in biomass. Inter-species differences in growth patterns were observed, with Rhizopus microsporus and Mucor spp. reaching the plateau of growth earlier compared to other species. These findings on the temporal relationship and inter-species differences of hyphal growth and metabolic activity for zygomycetes may be useful in the design and interpretation of in vitro studies of these emerging pathogens. |
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Authors:
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Charalampos Antachopoulos; Joseph Meletiadis; Emmanuel Roilides; Tin Sein; Deanna A Sutton; Brian L Wickes; Michael G Rinaldi; William G Merz; Yvonne R Shea; Thomas J Walsh |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: Medical mycology : official publication of the International Society for Human and Animal Mycology Volume: 44 ISSN: 1369-3786 ISO Abbreviation: Med. Mycol. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-02 Completed Date: 2006-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9815835 Medline TA: Med Mycol Country: England |
Other Details:
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Languages: eng Pagination: 429-38 Citation Subset: IM |
Affiliation:
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Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Humans Mucorales / growth & development*, metabolism* Mucormycosis / microbiology Nonlinear Dynamics Species Specificity Spectrophotometry Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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