Document Detail


Relationship between lymphangiogenesis and exudates during the wound-healing process of mouse skin full-thickness wound.
MedLine Citation:
PMID:  19614925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We considered the relationship among exudate, wound area, angiogenesis, lymphangiogenesis, and reepithelialization during wound healing. Full-thickness wounds were made on the dorsum of mice. The weight of exudate absorbed into the dressing as well as the wound area was determined daily. Sections of the wounds were stained with anti-LYVE-1 and CD31 antibodies. Indian ink was injected into the wound for observing the movement of the exudate on days 3, 5, and 7 after wounding. New epithelium completely covered the wound on day 11. The quantity of exudate peaked on day 1, and then rapidly decreased until it was undetectable on day 11. Most of the Indian ink injected into the wound was retained within the wound and did not flow into the surrounding tissue. New blood vessels showed a uniform distribution in the granulation tissue on day 5. New lymphatics appeared in the granulation tissue approximately 2 days later than the blood vessels and they were distributed toward the center of the granulation tissue on day 11. Thus, reduction of exudate from the wound appears to be related to blood vessels, not lymphatics. However, increasing lymphatics may play a role in the late phase of the wound-healing process.
Authors:
Kimi Shimamura; Toshio Nakatani; Aiko Ueda; Junko Sugama; Mayumi Okuwa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  17     ISSN:  1524-475X     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2009 Jul-Aug
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  598-605     Citation Subset:  IM    
Affiliation:
Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Kanazawa 9200942, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Exudates and Transudates / physiology*
Lymphangiogenesis / physiology*
Male
Mice
Mice, Inbred C57BL
Skin / injuries*,  pathology
Wound Healing / physiology*

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