| Relationship between levels of oxidative DNA damage, lipid peroxidation and mitochondrial membrane potential in young and old F344 rats. | |
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MedLine Citation:
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PMID: 16517504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The extent of in vivo oxidative damage has been known to be cumulative over the period of the life of mammals. Our hypothesis is that there should be a positive correlation between the levels of 8-hydroxy-2'-deoxyguanosine (8OHdG) and 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)) in major rat tissues. We also investigated whether increased level of oxidative stress causes a decrease in the mitochondrial membrane potential of peripheral lymphocytes of old rats using the MitoTracker Red fluorochrome. Our results show positive correlations between 8OHdG and 8-iso-PGF(2alpha) for liver, brain and kidney measured by HPLC-UV-ECD (electrochemical detector) and EIA methods, respectively. However, heart tissues show a negative correlation. The mitochondrial membrane potential of old rat lymphocytes records significant decrease compared with the young lymphocytes. Based on our results, we conclude that in ageing studies, specific tissues need to be examined in order to measure the localised DNA damage and lipid peroxidation as different tissues display different extent of oxidative damage. We believe this approach of using combined markers is useful to verify the true efficacy of health intervention studies in animals and humans. In addition, the isoprostane assay can be further developed looking at lipid peroxidation as a potential marker in ageing studies. |
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Authors:
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Yee Ting Wong; Runsheng Ruan; Francis Eng Hock Tay |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical research Volume: 40 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 393-402 Citation Subset: IM |
Affiliation:
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Institute of Bioengineering and Nanotechnology, Singapore, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging*
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genetics Animals Chromatography, High Pressure Liquid DNA Damage / physiology* Deoxyguanosine / analogs & derivatives, analysis Dinoprost / analogs & derivatives, analysis Immunoenzyme Techniques Lipid Peroxidation / physiology* Lymphocytes / metabolism Male Membrane Potentials / physiology* Mitochondria / metabolism* Oxidative Stress / physiology* Rats Rats, Inbred F344 |
| Chemical | |
Reg. No./Substance:
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0/8-hydroxy-2'-deoxyguanosine; 27415-26-5/8-epi-prostaglandin F2alpha; 551-11-1/Dinoprost; 961-07-9/Deoxyguanosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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