| Relationship between insulin sensitivity and sphingomyelin signaling pathway in human skeletal muscle. | |
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MedLine Citation:
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PMID: 15111489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vitro studies revealed that insulin resistance might be associated with the intracellular formation of ceramide, the second messenger in the sphingomyelin signaling pathway. The aim of the present study was to examine the content and composition of fatty acids in ceramide and sphingomyelin in human muscle and to evaluate their relationships with insulin sensitivity. The study was conducted on 27 male subjects with normal glucose tolerance. Euglycemic-hyperinsulinemic clamps and biopsies of vastus lateralis muscle were performed. In 10 subjects, additional biopsies were taken after a 4-h clamp and after a clamp with concurrent Intralipid/heparin infusion. We identified 13 ceramides and sphingomyelins according to fatty acid residues. Insulin sensitivity was related to total ceramide content (r = -0.49, P = 0.01) and to ceramide consisting of palmitic (r = -0.48, P = 0.011), palmitoleic (r = -0.45, P = 0.019), mirystic (r = -0.42, P = 0.028), and nervonic acid (r = -0.39, P = 0.047). Hyperinsulinemia did not affect estimated muscle parameters. Intralipid/heparin infusion resulted in a 24.73% decrease in insulin sensitivity (P = 0.007) and a 47.81% increase in ceramide content (P = 0.005). These changes were significantly related to each other (r = -0.64, P = 0.046). A relationship with the decrease in insulin sensitivity was also observed for ceramides consisting of palmitic (r = -0.68, P = 0.03) and linoleic (r = -0.66, P = 0.038) acid. Our data indicate that the sphingomyelin signaling pathway in muscle might be an important factor determining the development of insulin resistance in humans. |
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Authors:
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Marek Straczkowski; Irina Kowalska; Agnieszka Nikolajuk; Stella Dzienis-Straczkowska; Ida Kinalska; Marcin Baranowski; Malgorzata Zendzian-Piotrowska; Zofia Brzezinska; Jan Gorski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Diabetes Volume: 53 ISSN: 0012-1797 ISO Abbreviation: Diabetes Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-04-27 Completed Date: 2004-06-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372763 Medline TA: Diabetes Country: United States |
Other Details:
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Languages: eng Pagination: 1215-21 Citation Subset: AIM; IM |
Affiliation:
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Department of Endocrinology, Diabetology and Internal Medicine, Medical University Bialystok, M.C. Sklodowskiej 24a, 15-276 Bialystok, Poland. mstraczkowski@poczta.onet.pl |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anticoagulants / pharmacology Ceramides / metabolism Fat Emulsions, Intravenous / pharmacology Glucose Clamp Technique Heparin / pharmacology Humans Insulin Resistance / physiology* Male Muscle, Skeletal / drug effects, metabolism* Signal Transduction* Sphingomyelins / metabolism, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Ceramides; 0/Fat Emulsions, Intravenous; 0/Sphingomyelins; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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