Document Detail


Relationship between hyaluronic acid binding assay and outcome in ART: a pilot study.
MedLine Citation:
PMID:  20860626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sperm-hyaluronan binding assay (HBA) is a diagnostic kit for assessing sperm maturity, function and fertility. The aim of this prospective cohort pilot study was to evaluate the relationship between HBA and WHO sperm parameters (motility, concentration and detailed morphology) and possible influence of sperm processing on hyaluronic acid binding. A cohort of 68 patients undergoing a first combo in vitro fertilisation/intracytoplasmic sperm injection treatment after failure of three or more intrauterine insemination cycles were included in the study. Outcome measures studied were fertilisation rate, embryo quality, ongoing pregnancy rate and cumulative pregnancy rate. HBA outcome improved after sperm preparation and culture, but was not correlated to detailed sperm morphology, concentration or motility. HBA did not provide additional information for identifying patients with poor or absent fertilisation, although the latter had more immature sperm cells and cells with cytoplasmic retention present in their semen. HBA outcome in the neat sample was significantly correlated with embryo quality, with miscarriage rates and ongoing pregnancy rates in the fresh cycles, but not with the cumulative ongoing pregnancy rate. No threshold value for HBA and outcome in combo IVF/ICSI treatment could be established. The clinical value for HBA in addition to routine semen analysis for this patient population seems limited.
Authors:
Martine Nijs; E Creemers; A Cox; M Janssen; E Vanheusden; J Van der Elst; W Ombelet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Andrologia     Volume:  42     ISSN:  1439-0272     ISO Abbreviation:  Andrologia     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-23     Completed Date:  2011-01-10     Revised Date:  2011-12-20    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  291-6     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Verlag GmbH.
Affiliation:
Genk Institute for Fertility Technology, Ziekenhuis Oost Limburg, Genk, Belgium. martine.nijs@zol.be
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MeSH Terms
Descriptor/Qualifier:
Adult
Cohort Studies
Female
Humans
Hyaluronic Acid / chemistry*
Male
Middle Aged
Pilot Projects
Pregnancy
Pregnancy Rate
Prospective Studies
Reagent Kits, Diagnostic*
Reproductive Techniques, Assisted*
Sperm Count*
Sperm Injections, Intracytoplasmic
Sperm Motility*
Spermatozoa / cytology*
Treatment Outcome
Young Adult
Chemical
Reg. No./Substance:
0/Reagent Kits, Diagnostic; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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