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Relationship between expression of sex steroid receptors and structure of the seminal vesicles after neonatal treatment of rats with potent or weak estrogens.
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MedLine Citation:
PMID:  11748029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we evaluated the effect of manipulating the estrogen and androgen environment of the neonatal male rat on subsequent immunoexpression of sex steroid receptors in the seminal vesicles (SVs) at age 18 days. The aim was to establish to what extent such changes were associated with and predictive of changes in SV structure/composition. Treatments were either diethylstilbestrol (DES; 10, 1, or 0.1 microg/injection), ethinyl estradiol (EE; 10 microg/injection), tamoxifen (2 mg/kg/day), flutamide (50 mg/kg), a gonadotropin-releasing hormone antagonist (GnRHa; 10 mg/kg), genistein (4 mg/kg/day), octylphenol (2 mg/injection), or bisphenol A (0.5 mg/injection). Compared with controls, treatment with DES (10 microg) induced loss of epithelial and stromal androgen receptor (AR) immunoexpression coincident with induction of stromal progesterone receptor (PR) immunoexpression and upregulation of stromal immunoexpression of estrogen receptor-alpha (ERalpha). These changes were associated with gross distortion (increase) of the normal stromal:epithelial tissue proportions in the SVs. DES (1 microg) and EE induced similar but less pronounced changes, and DES (0.1 microg) had no noticeable effect. Tamoxifen and flutamide induced PR and slightly upregulated ERalpha immunoexpression but had only a minor or no effect on AR expression and the stromal:epithelial ratio, though flutamide retarded normal development of the SVs. The latter was also evident in GnRHa-treated males, but otherwise this treatment had no effect on AR and PR immunoexpression. None of the foregoing treatments had any detectable effect on the immunoexpression of ERss in stromal or epithelial cells. The major treatment-induced changes in immunoexpression of AR, PR, and ERalpha and lack of change in ERss were confirmed by Western blots of SV protein extracts. None of the three weak (environmental) estrogens tested caused any detectable change in sex steroid receptor immunoexpression or SV tissue composition. We conclude that treatment-induced loss of AR is a prerequisite for altered stromal:epithelial proportions in the SVs and that such loss is always associated with induction of PR and upregulation of ERalpha; the latter two changes are insufficient on their own to bring about such a change. Nevertheless, induction of PR expression was always associated with altered SV development and is a potentially useful marker because it is not normally expressed in male reproductive tissues.
Authors:
K Williams; J S Fisher; K J Turner; C McKinnell; P T Saunders; R M Sharpe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental health perspectives     Volume:  109     ISSN:  0091-6765     ISO Abbreviation:  Environ. Health Perspect.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-18     Completed Date:  2002-02-05     Revised Date:  2013-04-29    
Medline Journal Info:
Nlm Unique ID:  0330411     Medline TA:  Environ Health Perspect     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1227-35     Citation Subset:  IM    
Affiliation:
MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, Edinburgh, Scotland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Biological Markers / analysis
Environmental Pollutants / adverse effects*
Estrogens / pharmacology*
Gene Expression Regulation*
Male
Rats
Rats, Wistar
Receptors, Steroid / biosynthesis*
Seminal Vesicles / drug effects,  ultrastructure*
Up-Regulation
Grant Support
ID/Acronym/Agency:
MC_U127685844//Medical Research Council; U.1276.00.002.00005.01 (85844)//Medical Research Council
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Environmental Pollutants; 0/Estrogens; 0/Receptors, Steroid
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Environ Health Perspect
ISSN: 0091-6765
Article Information
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Print publication date: Month: 12 Year: 2001
Volume: 109 Issue: 12
First Page: 1227 Last Page: 1235
ID: 1240504
PubMed Id: 11748029
Publisher Item Identifier: sc271_5_1835

Relationship between expression of sex steroid receptors and structure of the seminal vesicles after neonatal treatment of rats with potent or weak estrogens.
K Williams
J S Fisher
K J Turner
C McKinnell
P T Saunders
R M Sharpe
MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, Edinburgh, Scotland.


Article Categories:
  • Research Article


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