Document Detail


Relationship between cerebral blood flow and oxygen metabolism, and extracellular glucose and lactate concentrations during middle cerebral artery occlusion and reperfusion: a microdialysis and positron emission tomography study in nonhuman primates.
MedLine Citation:
PMID:  16028767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECT: Changes in lactate and glucose levels in the brain may be used to monitor a dynamic ischemic process. The authors related extracellular concentrations of glucose and lactate to regional cerebral blood flow (rCBF) and cerebral metabolic rate of oxygen (CMRO2) in a model of ischemia. METHODS: Transient (2 hours) middle cerebral artery occlusion (MCAO) was performed in eight macaque monkeys. Extracellular measurements of glucose and lactate levels using microdialysis (two probes in each brain) and sequential positron emission tomography measurements were performed during MCAO and reperfusion. Glucose and lactate levels were related to rCBF and CMRO2 as well as the pathophysiological categories of severe ischemia and penumbra. In probe regions characterized by severe ischemia, there were significant changes in glucose and lactate levels and the lactate/glucose ratio. In probe regions distinguished by penumbra, only lactate levels increased significantly and, in general, the changes were smaller and transient. This difference between severe ischemic and penumbral regions was significant for all microdialysis parameters. There was a significant correlation between glucose, and CBF and CMRO2. Lactate concentration was correlated with CMRO2. CONCLUSIONS: Extracellular glucose levels might be limited by rCBF, whereas lactate levels were more related to CMRO2. Lactate concentration is a better marker of irreversible ischemia than glucose concentration, although near-zero levels of glucose during MCAO probably signals near-complete cessation of rCBF. In situations with elevated lactate levels, glucose may help to discriminate between partial and severe ischemia.
Authors:
Peter Frykholm; Lars Hillered; Bengt Långström; Lennart Persson; Johann Valtysson; Per Enblad
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurosurgery     Volume:  102     ISSN:  0022-3085     ISO Abbreviation:  J. Neurosurg.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-20     Completed Date:  2005-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0253357     Medline TA:  J Neurosurg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1076-84     Citation Subset:  AIM; IM    
Affiliation:
Department of Clinical Neurosciences/Neurosurgery, Uppsala University Hospital, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebrovascular Circulation / physiology*
Disease Models, Animal
Extracellular Space / metabolism
Glucose / metabolism*
Infarction, Middle Cerebral Artery / metabolism*,  physiopathology,  radionuclide imaging
Lactic Acid / metabolism*
Macaca mulatta
Microdialysis
Oxygen / metabolism*
Positron-Emission Tomography*
Reperfusion Injury / metabolism,  physiopathology,  radionuclide imaging
Chemical
Reg. No./Substance:
50-21-5/Lactic Acid; 50-99-7/Glucose; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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