| Relationship between cell proliferation, cell-cycle phase, and retroviral vector production in FLYRD18 human packaging cells. | |
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MedLine Citation:
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PMID: 11400106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The relatively low concentrations of retroviral vectors produced by most packaging cells requires the optimization and intensification of their production to make a commercially viable product for gene therapy. While a number of reports exist concerning target cell-cycle effects on retroviral vector infection efficiency, no studies have been reported on the effects of packaging cell cycle on vector production. We have studied the effect of proliferation of the human packaging cell line, FLYRD18, on vector production. In addition, the titer levels of vector produced by cells in each phase of the cell cycle were compared. Numerous studies suggested progression of the cells through the cell cycle to be essential for vector production. However, vector release was found not to be predominant in any particular phase of the cell cycle. These findings indicate that packaging cell proliferation is important for optimal virus production and that arrest of the cells in any particular phase of the cell cycle affords no benefits in retroviral vector production. In contrast to previous reports (using other cell lines), we observed no temporary inhibition of cell cycle progression after detachment of cells from their substratum and that virus production occurred immediately after re-plating of the cells. The findings in this report are important for determining the optimal culture conditions for vector production by packaging cells in vitro. |
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Authors:
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S McTaggart; M Al-Rubeai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 76 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2001 |
Date Detail:
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Created Date: 2001-06-11 Completed Date: 2001-09-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 52-60 Citation Subset: IM |
Copyright Information:
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Copyright 2001 John Wiley & Sons, Inc. |
Affiliation:
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Animal Cell Technology Group, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Count Cell Culture Techniques / methods Cell Cycle / drug effects, physiology* Cell Division / physiology Cell Line Cell Separation / methods Culture Media Demecolcine / pharmacology Fibrosarcoma Flow Cytometry / methods Genetic Vectors* Humans Retroviridae / genetics, physiology* Thymidine / metabolism Tumor Cells, Cultured Virus Replication* / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 477-30-5/Demecolcine; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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