Document Detail


Relationship between cell differentiation and binding of fluorescein isothiocyanate (FITC)-conjugated insulin of keratinocytes in culture.
MedLine Citation:
PMID:  2439607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Basal-type keratinocytes, isolated from newborn rat skin and separated on Percoll density gradients, proliferate in low (0.1 mM) calcium medium and, after raising the calcium level to normal (1.96 mM), stratify. Cells in the low calcium culture do not have extensive cell-cell connections, as seen with fluorescein isothiocyanate (FITC)-conjugated insulin. Fluorescein isothiocyanate-conjugated concanavalin A and Griffonia simplicifolia isolectin B4, but not peanut agglutinin (PNA), fluorescently label these cells. In 3-day-old low calcium cultures, within 2 h after raising the calcium of the medium to the normal level, intense binding of PNA to cells appears and neighboring cells are connected through bundles of filaments that are fluorescently labeled by FITC-insulin. After 2 days in normal calcium medium, the cultures exhibit relatively smooth, straightlined, cell boundaries that are labeled by FITC-insulin and cell boundaries and intracellular granules that are stained by hematoxylin. One day later, similar cell boundaries are present, but they are not significantly decorated by FITC-insulin and, under phase contrast microscopy, are dark. Free FITC gives labeling patterns similar to those given by FITC-insulin, but the FITC labeling is blocked by mercaptoethanol and dithiothreitol in contrast to FITC-insulin binding. The present results suggest the insulin moiety is involved in the labeling by FITC-insulin and the labeling is chronologically related to the stage of cell differentiation.
Authors:
H W Feng; X P Jin; I A Bernstein
Related Documents :
9419397 - Calcium and colorectal epithelial cell proliferation in ulcerative colitis.
657277 - Metabolically stable non-histone chromosomal proteins in growing maize roots.
16481917 - Gadolinium-fullerenol as a paramagnetic contrast agent for cellular imaging.
12482717 - The origin of mesoderm in phoronids.
21900887 - Prolonged use of aspirin alters human and rat intestinal cells and thereby limits the a...
24186937 - Mechanism and physiological significance of growth factor-related autophagy.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  89     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-07-28     Completed Date:  1987-07-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  73-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / pharmacology
Cell Differentiation
Cells, Cultured
Concanavalin A / analogs & derivatives,  metabolism
Culture Media
Epidermis / cytology*,  metabolism
Fluorescein-5-isothiocyanate* / analogs & derivatives*
Fluoresceins / metabolism
Insulin / analogs & derivatives*,  metabolism,  pharmacology
Insulin Antagonists
Keratins*
Lectins / metabolism
Grant Support
ID/Acronym/Agency:
AM07236/AM/NIADDK NIH HHS; AM15206/AM/NIADDK NIH HHS; CA32470/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fluoresceins; 0/Insulin Antagonists; 0/Lectins; 0/fluorescein isothiocyanate-concanavalin A; 0/insulin, fluorescein-isothiocyanated-; 11028-71-0/Concanavalin A; 11061-68-0/Insulin; 3326-32-7/Fluorescein-5-isothiocyanate; 68238-35-7/Keratins; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibition of the proliferation of transformed epidermal cells in culture by various prostaglandins.
Next Document:  Lichenoid tissue reaction (LTR) induced by local transfer of Ia-reactive T-cell clones. II. LTR by e...