Document Detail


Relationship between bovine oocyte morphology and in vitro developmental potential.
MedLine Citation:
PMID:  16700976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the relationship between the morphology of oocytes collected from small antral follicles and their developmental capacity. Immature oocytes were classified into seven groups and cultured in vitro for maturation (IVM), fertilization (IVF) and development to blastocysts (IVC). After IVF, sperm penetration and normal fertilization rates were higher in the oocytes whose cytoplasm appeared brown. The rate of polyspermy was highest in the oocytes whose cytoplasm was black. After IVC, the rates of cleavage and of development to the blastocyst stage were also higher in the brown oocytes. Although the oocytes with dark clusters in a pale cytoplasm showed lower cleavage rates, cleaved zygotes had high developmental rates the same as the oocytes with a brown cytoplasm. Transmission electron microscopy showed that the oocytes with a pale or black cytoplasm had organelles arranged differently from other oocytes before IVM. Most of the oocytes with a brown and homogeneous cytoplasm or small diameter had the characteristics of immature cytoplasm (large clusters of cortical granules) even after IVM. On the other hand, the brown oocytes with a dark zone at the periphery or with dark clusters showed the same arrangement of organelles as in vivo matured oocytes. The oocytes with a pale or black cytoplasm appeared to be degenerating and/or ageing. In conclusion, a dark ooplasm indicates an accumulation of lipids and good developmental potential, while a light-coloured ooplasm indicates a low density of organelles and poor developmental potential. A black ooplasm indicates ageing and low developmental potential.
Authors:
Masashi Nagano; Seiji Katagiri; Yoshiyuki Takahashi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  14     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-05-16     Completed Date:  2006-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  53-61     Citation Subset:  IM    
Affiliation:
Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle / embryology*
Female
Fertilization in Vitro / veterinary
Microscopy, Electron, Transmission
Oocytes / growth & development*,  ultrastructure*

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