| Relationship between arachidonic acid metabolism, myeloperoxidase activity and leukocyte infiltration in a rat model of inflammatory bowel disease. | |
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MedLine Citation:
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PMID: 2847506 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The relationship between 14C-arachidonic acid (14C-AA) metabolism, myeloperoxidase activity (MPO) and leukocyte infiltration was studied in a chronic model of inflammatory bowel disease, induced by a single intrarectal application of the hapten, trinitrobenzene sulphonic acid (TNB). The colonic damage produced by TNB was accompanied, after 12-36 hours, by a marked increase in MPO, which was directly correlated to leukocyte infiltration, assessed histologically. There was also a marked increase in the metabolism of 14C-AA, by homogenates of inflamed colon, to 12-, 15-HETE and 6-keto-PGF1 alpha as indices of lipoxygenase and cyclo-oxygenase metabolism respectively. However, a further increase in MPO-cellular infiltration, between 36-72 hours after TNB, was accompanied by a reduction in 12- and 15-HETE formation. The increase in MPO-cellular infiltration was maintained for up to 3 weeks, at which time both 12-, 15-HETE and 6-keto-PGF1 alpha formation had returned to control levels. These results suggest that these AA metabolites have a greater importance in the acute phase of the inflammatory response induced by TNB compared to the later chronic phase. |
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Authors:
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N K Boughton-Smith; J L Wallace; B J Whittle |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Agents and actions Volume: 25 ISSN: 0065-4299 ISO Abbreviation: Agents Actions Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1988-11-28 Completed Date: 1988-11-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0213341 Medline TA: Agents Actions Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 115-23 Citation Subset: IM |
Affiliation:
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Department of Mediator Pharmacology, Wellcome Research Laboratories, Beckenham, Kent, U.K. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism* Colon / metabolism, pathology Female Hydroxyeicosatetraenoic Acids / biosynthesis Inflammatory Bowel Diseases / chemically induced, metabolism*, pathology Leukocytes / metabolism*, pathology Peroxidase / metabolism* Rats Rats, Inbred Strains Trinitrobenzenesulfonic Acid |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Hydroxyeicosatetraenoic Acids; 2508-19-2/Trinitrobenzenesulfonic Acid; 506-32-1/Arachidonic Acid; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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